Effect of Two Freezing Extenders on Characteristic of Fresh and Frozen-Thawed Semen in Endangered Old Kladruber Stallions - A Pilot Study
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A73482" target="_blank" >RIV/60460709:41210/17:73482 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.17221/76/2016-CJAS" target="_blank" >http://dx.doi.org/10.17221/76/2016-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/76/2016-CJAS" target="_blank" >10.17221/76/2016-CJAS</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of Two Freezing Extenders on Characteristic of Fresh and Frozen-Thawed Semen in Endangered Old Kladruber Stallions - A Pilot Study
Popis výsledku v původním jazyce
The aim of the study was to evaluate the effect of two different extenders on sperm characteristic before equilibration and post-thaw in the endangered Old Kladruber stallions. Also, the response of individual stallions to the extenders used was tested. Semen was collected from six stallions every other day within one week. After centrifugation of the collected sperm-rich fraction, the supernatant was removed and sperm pellets were divided to two aliquots, these were diluted either with Gent (Minitube, Germany) or privately manufactured lactose-EDTA-egg yolk extender (Lact). Three cryopreserved insemination doses (IDs) from each extender (Gent and Lact) were prepared for each stallion from one collection (108 samples from six stallions in total). As a parameter of quality, the motility (computer assisted sperm analysis), viability (fluorescence staining), and morphology (eosin/nigrosine staining) were evaluated after dilution with freezing extenders (fresh) and after thawing (frozen-thawed. The diffe
Název v anglickém jazyce
Effect of Two Freezing Extenders on Characteristic of Fresh and Frozen-Thawed Semen in Endangered Old Kladruber Stallions - A Pilot Study
Popis výsledku anglicky
The aim of the study was to evaluate the effect of two different extenders on sperm characteristic before equilibration and post-thaw in the endangered Old Kladruber stallions. Also, the response of individual stallions to the extenders used was tested. Semen was collected from six stallions every other day within one week. After centrifugation of the collected sperm-rich fraction, the supernatant was removed and sperm pellets were divided to two aliquots, these were diluted either with Gent (Minitube, Germany) or privately manufactured lactose-EDTA-egg yolk extender (Lact). Three cryopreserved insemination doses (IDs) from each extender (Gent and Lact) were prepared for each stallion from one collection (108 samples from six stallions in total). As a parameter of quality, the motility (computer assisted sperm analysis), viability (fluorescence staining), and morphology (eosin/nigrosine staining) were evaluated after dilution with freezing extenders (fresh) and after thawing (frozen-thawed. The diffe
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
—
Svazek periodika
62
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
7
Strana od-do
227-233
Kód UT WoS článku
000403547200001
EID výsledku v databázi Scopus
2-s2.0-85020783738