Semen freezability of endangered Old Kladruber horse – a pilot study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3AN0000012" target="_blank" >RIV/60460709:41210/16:N0000012 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Semen freezability of endangered Old Kladruber horse – a pilot study

Popis výsledku v původním jazyce

The aim of this study was to evaluate sperm characteristics of Old Kladruber stallions cryopreserved in two packaging systems. Each stallion was collected three times and samples were pre diluted and centrifuged (650 x g/15 min). Afterwards, Gent extender (MiniTube, Germany) with 20 percent (v/v) of egg yolk was added to obtain a final concentration at least 250 x 106 of progressively motile spermatozoa/ID. Extended semen was loaded either into 0,5 ml plastic straws or into 5 ml aluminum tubes. Horizontal freezing was performed in a styrofoam box (Animal Reproduction systems, Chino CA, USA). Motility was measured using CASA (Nis Elements, ver. 4,30, Laboratory Imaging, Prague, Czech Republic). The following kinematic parameters of sperm motility were evaluated: curvilinear velocity (VCL, um/s), straight line velocity (VSL, um/s) and average path velocity (VAP, um/s). Viability was evaluated using carboxyfluorescein diacetate and propidium iodide. The incidence of apoptotic sperms was evaluated with Yo Pro1 staining using propidium iodide and DAPI. Ten insemination doses from each stallion (5x 0,5 ml straws and 5x 5 ml aluminum tubes) were evaluated after thawing.

Název v anglickém jazyce

Semen freezability of endangered Old Kladruber horse – a pilot study

Popis výsledku anglicky

The aim of this study was to evaluate sperm characteristics of Old Kladruber stallions cryopreserved in two packaging systems. Each stallion was collected three times and samples were pre diluted and centrifuged (650 x g/15 min). Afterwards, Gent extender (MiniTube, Germany) with 20 percent (v/v) of egg yolk was added to obtain a final concentration at least 250 x 106 of progressively motile spermatozoa/ID. Extended semen was loaded either into 0,5 ml plastic straws or into 5 ml aluminum tubes. Horizontal freezing was performed in a styrofoam box (Animal Reproduction systems, Chino CA, USA). Motility was measured using CASA (Nis Elements, ver. 4,30, Laboratory Imaging, Prague, Czech Republic). The following kinematic parameters of sperm motility were evaluated: curvilinear velocity (VCL, um/s), straight line velocity (VSL, um/s) and average path velocity (VAP, um/s). Viability was evaluated using carboxyfluorescein diacetate and propidium iodide. The incidence of apoptotic sperms was evaluated with Yo Pro1 staining using propidium iodide and DAPI. Ten insemination doses from each stallion (5x 0,5 ml straws and 5x 5 ml aluminum tubes) were evaluated after thawing.

Druh

O - Ostatní výsledky

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů