Impact of BCS of dairy cows on quality of embryos
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F13%3A%230000594" target="_blank" >RIV/49608851:_____/13:#0000594 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Impact of BCS of dairy cows on quality of embryos
Popis výsledku v původním jazyce
The aim of the study was to examine quality of in vivo-derived cattle embryos. The embryos were recovered from superovulated Holstein cows (n = 59), classified for body condition score (BCS) according to a 5-point scale (BCS2- n = 17; BCS3- n = 33; BCS4-n = 9), on 6th day after the first insemination by a non-surgical flushing of the uterine horns. The embryos were morphologically evaluated for developmental stage (morula, blastocyst, fragmented/degenerated) and analyzed for apoptosis (TUNEL), total cell number (DAPI), actin cytoskeleton quality (phalloidine-TRITC) and cell ultrastructure (transmission electron microscopy). The highest embryo yield (69.3%) was obtained in the BCS3 group, but with the BCS elevation the embryo yield decreased and the last embryo yield (35%) was obtained in the BCS4. The lower embryo cell count and higher number of embryos with cytoplasmic fragmentation were recorded in the BCS4, compared to BCS2 or BCS3 groups. Apoptotic index was highest in the BCS4 em
Název v anglickém jazyce
Impact of BCS of dairy cows on quality of embryos
Popis výsledku anglicky
The aim of the study was to examine quality of in vivo-derived cattle embryos. The embryos were recovered from superovulated Holstein cows (n = 59), classified for body condition score (BCS) according to a 5-point scale (BCS2- n = 17; BCS3- n = 33; BCS4-n = 9), on 6th day after the first insemination by a non-surgical flushing of the uterine horns. The embryos were morphologically evaluated for developmental stage (morula, blastocyst, fragmented/degenerated) and analyzed for apoptosis (TUNEL), total cell number (DAPI), actin cytoskeleton quality (phalloidine-TRITC) and cell ultrastructure (transmission electron microscopy). The highest embryo yield (69.3%) was obtained in the BCS3 group, but with the BCS elevation the embryo yield decreased and the last embryo yield (35%) was obtained in the BCS4. The lower embryo cell count and higher number of embryos with cytoplasmic fragmentation were recorded in the BCS4, compared to BCS2 or BCS3 groups. Apoptotic index was highest in the BCS4 em
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
GI - Šlechtění a plemenářství hospodářských zvířat
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/QI91A061" target="_blank" >QI91A061: Vliv rozdílné metabolické aktivity organismu na biologické a oplodňovací charakteristiky gamet dojeného skotu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů