Impact of BCS of dairy cows on quality of embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F13%3A63344" target="_blank" >RIV/60460709:41210/13:63344 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Impact of BCS of dairy cows on quality of embryos

Popis výsledku v původním jazyce

The aim of the study was to examine quality of in vivo-derived cattle embryos. The embryos were recovered from superovulated Holstein cows (n = 59), classified for body condition score (BCS) according to a 5-point scale (BCS2- n = 17; BCS3- n = 33; BCS4-n = 9), on 6th day after the first insemination by a non-surgical flushing of the uterine horns. The embryos were morphologically evaluated for developmental stage (morula, blastocyst, fragmented/degenerated) and analyzed for apoptosis (TUNEL), total cell number (DAPI), actin cytoskeleton quality (phalloidine-TRITC) and cell ultrastructure (transmission electron microscopy). The highest embryo yield (69.3%) was obtained in the BCS3 group, but with the BCS elevation the embryo yield decreased and the least embryo yield (35%) was obtained in the BCS4. The lower embryo cell count and higher number of embryos with cytoplasmic fragmentation were recorded in the BCS4, compared to BCS2 or BCS3 groups. Apoptotic index was highest in the BCS4 e

Název v anglickém jazyce

Impact of BCS of dairy cows on quality of embryos

Popis výsledku anglicky

The aim of the study was to examine quality of in vivo-derived cattle embryos. The embryos were recovered from superovulated Holstein cows (n = 59), classified for body condition score (BCS) according to a 5-point scale (BCS2- n = 17; BCS3- n = 33; BCS4-n = 9), on 6th day after the first insemination by a non-surgical flushing of the uterine horns. The embryos were morphologically evaluated for developmental stage (morula, blastocyst, fragmented/degenerated) and analyzed for apoptosis (TUNEL), total cell number (DAPI), actin cytoskeleton quality (phalloidine-TRITC) and cell ultrastructure (transmission electron microscopy). The highest embryo yield (69.3%) was obtained in the BCS3 group, but with the BCS elevation the embryo yield decreased and the least embryo yield (35%) was obtained in the BCS4. The lower embryo cell count and higher number of embryos with cytoplasmic fragmentation were recorded in the BCS4, compared to BCS2 or BCS3 groups. Apoptotic index was highest in the BCS4 e

Druh

D - Stať ve sborníku

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QI91A061" target="_blank" >QI91A061: Vliv rozdílné metabolické aktivity organismu na biologické a oplodňovací charakteristiky gamet dojeného skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

17th Annual Conference of the European-Society-of-Domestic-Animal-Reproduction (ESDAR), Reproduction in Domestic Animals, 48 (Suppl. 1), Poster presentation, P144

ISBN

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ISSN

0936-6768

e-ISSN

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Počet stran výsledku

1

Strana od-do

107-107

Název nakladatele

WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA

Místo vydání

Bologna, ITALY

Místo konání akce

Bologna, ITALY

Datum konání akce

12. 9. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000323318700201