Cellular force microscopy for in vivo measurements of plant tissue mechanics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23520%2F12%3A43915629" target="_blank" >RIV/49777513:23520/12:43915629 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1104/pp.111.191460" target="_blank" >http://dx.doi.org/10.1104/pp.111.191460</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1104/pp.111.191460" target="_blank" >10.1104/pp.111.191460</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cellular force microscopy for in vivo measurements of plant tissue mechanics

Popis výsledku v původním jazyce

Although growth and morphogenesis are controlled by genetics, physical shape change in plant tissue results from a balance between cell wall loosening and intracellular pressure. Despite recent work demonstrating a role for mechanical signals in morphogenesis, precise measurement of mechanical properties at the individual cell level remains a technical challenge. To address this challenge we have developed Cellular Force Microscopy (CFM) that combines the versatility of classical micro-indentation techniques with high automation and resolution approaching that of atomic force microscopy. CFM's large range of forces provides the possibility to map the apparent stiffness of both plasmolysed and turgid tissue, as well as perform micro-puncture of cells using very high stresses. CFM experiments reveal that, within a tissue, local stiffness measurements can vary with the level of turgor pressure in an unexpected way. Altogether, our results highlight the importance of detailed physically ba

Název v anglickém jazyce

Cellular force microscopy for in vivo measurements of plant tissue mechanics

Popis výsledku anglicky

Although growth and morphogenesis are controlled by genetics, physical shape change in plant tissue results from a balance between cell wall loosening and intracellular pressure. Despite recent work demonstrating a role for mechanical signals in morphogenesis, precise measurement of mechanical properties at the individual cell level remains a technical challenge. To address this challenge we have developed Cellular Force Microscopy (CFM) that combines the versatility of classical micro-indentation techniques with high automation and resolution approaching that of atomic force microscopy. CFM's large range of forces provides the possibility to map the apparent stiffness of both plasmolysed and turgid tissue, as well as perform micro-puncture of cells using very high stresses. CFM experiments reveal that, within a tissue, local stiffness measurements can vary with the level of turgor pressure in an unexpected way. Altogether, our results highlight the importance of detailed physically ba

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JJ - Ostatní materiály

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT PHYSIOLOGY

ISSN

0032-0889

e-ISSN

—

Svazek periodika

158

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1514-1522

Kód UT WoS článku

000303001400003

EID výsledku v databázi Scopus

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