Combined model of bladder detrusor smooth muscle and interstitial cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23640%2F16%3A43929503" target="_blank" >RIV/49777513:23640/16:43929503 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/16:10324245 RIV/49777513:23520/16:43929503

Výsledek na webu

<a href="http://dx.doi.org/10.1177/1535370216655402" target="_blank" >http://dx.doi.org/10.1177/1535370216655402</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/1535370216655402" target="_blank" >10.1177/1535370216655402</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combined model of bladder detrusor smooth muscle and interstitial cells

Popis výsledku v původním jazyce

Understanding the interactions between the detrusor smooth muscle cells and other bladder cell types (e.g. interstitial cells, IC) that may significantly contribute to coordinating and modulating detrusor contractions represents a considerable challenge. Computer modeling could help to elucidate some properties that are difficult to address experimentally; therefore, we developed in silico models of detrusor smooth muscle cell and interstitial cells, coupled through gap junctions. The models include all of the major ion conductances and transporters described in smooth muscle cell and interstitial cells in the literature. The model of normal detrusor muscle (smooth muscle cell and interstitial cells coupled through gap junctions) completely reproduced the experimental results obtained with detrusor strips in the presence of several pharmacological interventions (ryanodine, caffeine, nimodipine), whereas the model of smooth muscle cell alone (without interstitial cells) failed to reproduce the experimental results. Next, a model of overactive bladder, a highly prevalent clinical condition in both men and women with increasing incidence at older ages, was produced by modifying several processes as reported previously: a reduction of Ca2ţ-release through ryanodine receptors and a reduction of Ca2ţ-dependent Kţ-conductance with augmented gap junctional coupling. This model was also able to reproduce the pharmacological modulation of overactive bladder. The results indicate that the non-smooth muscle cells of the detrusor (interstitial cells) contribute significantly to the contractile behavior of bladder detrusor muscle and should not be neglected. The model suggests that reduced Ca2ţ- release through ryanodine receptors and Ca2ţ-dependent Kţ-conductance together with augmented gap junctional coupling might play a major role in overactive bladder pathogenesis.

Název v anglickém jazyce

Combined model of bladder detrusor smooth muscle and interstitial cells

Popis výsledku anglicky

Understanding the interactions between the detrusor smooth muscle cells and other bladder cell types (e.g. interstitial cells, IC) that may significantly contribute to coordinating and modulating detrusor contractions represents a considerable challenge. Computer modeling could help to elucidate some properties that are difficult to address experimentally; therefore, we developed in silico models of detrusor smooth muscle cell and interstitial cells, coupled through gap junctions. The models include all of the major ion conductances and transporters described in smooth muscle cell and interstitial cells in the literature. The model of normal detrusor muscle (smooth muscle cell and interstitial cells coupled through gap junctions) completely reproduced the experimental results obtained with detrusor strips in the presence of several pharmacological interventions (ryanodine, caffeine, nimodipine), whereas the model of smooth muscle cell alone (without interstitial cells) failed to reproduce the experimental results. Next, a model of overactive bladder, a highly prevalent clinical condition in both men and women with increasing incidence at older ages, was produced by modifying several processes as reported previously: a reduction of Ca2ţ-release through ryanodine receptors and a reduction of Ca2ţ-dependent Kţ-conductance with augmented gap junctional coupling. This model was also able to reproduce the pharmacological modulation of overactive bladder. The results indicate that the non-smooth muscle cells of the detrusor (interstitial cells) contribute significantly to the contractile behavior of bladder detrusor muscle and should not be neglected. The model suggests that reduced Ca2ţ- release through ryanodine receptors and Ca2ţ-dependent Kţ-conductance together with augmented gap junctional coupling might play a major role in overactive bladder pathogenesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

EXPERIMENTAL BIOLOGY AND MEDICINE

ISSN

1535-3702

e-ISSN

—

Svazek periodika

241

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

1853-1864

Kód UT WoS článku

000383439000017

EID výsledku v databázi Scopus

2-s2.0-84988014662