Fusarium Identification by PCR and DON Content in Grain of Ancient Wheat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F11%3A43882059" target="_blank" >RIV/60076658:12220/11:43882059 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fusarium Identification by PCR and DON Content in Grain of Ancient Wheat

Popis výsledku v původním jazyce

Fusarium infestation and DON content were studied in genetic resources of spring wheat (einkorn, emmer wheat, spelt wheat, intermediate forms of bread wheat). The study aimed at the comparison of grain contamination rates in various wheat species being grown in organic farming systems. The trials were established on certified organic parcels in two different localities in the Czech Republic in 2009 and 2010. The PCR method and specific primers were used to detect Fusarium. The proportion of deoxynivalenol (DON) was indicated and measured by the immuno-affinity chromatography (ROSA(R) DON Quantitative Test). The grain DON contamination rate (1.25 mg/kg) was set up in compliance with the European Commission (EC) Regulation No. 1126/2007. This regulationsets up limits that have never been exceeded in any of the varieties. The strongest contamination rate was identified in grains of the control bread wheat varieties, and in the SW Kadrilj cultivar (0.98 mg/kg) being grown in Ceske Budejov

Název v anglickém jazyce

Fusarium Identification by PCR and DON Content in Grain of Ancient Wheat

Popis výsledku anglicky

Fusarium infestation and DON content were studied in genetic resources of spring wheat (einkorn, emmer wheat, spelt wheat, intermediate forms of bread wheat). The study aimed at the comparison of grain contamination rates in various wheat species being grown in organic farming systems. The trials were established on certified organic parcels in two different localities in the Czech Republic in 2009 and 2010. The PCR method and specific primers were used to detect Fusarium. The proportion of deoxynivalenol (DON) was indicated and measured by the immuno-affinity chromatography (ROSA(R) DON Quantitative Test). The grain DON contamination rate (1.25 mg/kg) was set up in compliance with the European Commission (EC) Regulation No. 1126/2007. This regulationsets up limits that have never been exceeded in any of the varieties. The strongest contamination rate was identified in grains of the control bread wheat varieties, and in the SW Kadrilj cultivar (0.98 mg/kg) being grown in Ceske Budejov

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

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Projekt

<a href="/cs/project/QH82272" target="_blank" >QH82272: Využití jarních forem vybraných druhů pšenice v ekologickém zemědělství</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food, Agriculture & Environment

ISSN

1459-0255

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

FI - Finská republika

Počet stran výsledku

4

Strana od-do

321-325

Kód UT WoS článku

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EID výsledku v databázi Scopus

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