Computer-Assisted Sperm Analysis of Head Morphometry and Kinematic Parameters in Warmblood Stallions Spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F17%3A43895881" target="_blank" >RIV/60076658:12220/17:43895881 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jevs.2017.05.012" target="_blank" >http://dx.doi.org/10.1016/j.jevs.2017.05.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jevs.2017.05.012" target="_blank" >10.1016/j.jevs.2017.05.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Computer-Assisted Sperm Analysis of Head Morphometry and Kinematic Parameters in Warmblood Stallions Spermatozoa

Popis výsledku v původním jazyce

The present study was conducted to determine the seminal characteristics of warmblooded stallions and to assess a relationship between motility parameters of spermatozoa and sperm head dimensions using computer-assisted sperm analysis. In total, 35 ejaculates were collected during the breeding season 2016 from 10 clinically healthy and fertile warmblooded stallions (age 3?22 years). The volume of ejaculate (mL), sperm concentration (M/mL), motility of sperm with kinematic parameters, and sperm morphometry were evaluated. The motility characteristics, sperm concentration, and morphometric parameters of sperm were determined objectively using Sperm Class Analyzer (SCA, MICROPTIC SL). The overall mean ? standard deviation values for volume of ejaculate, concentration, total motility (MOT), progressive motility (PMOT), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), straightness (STR), linearity (LIN), amplitude of lateral head displacement (ALH), beat-cross frequency (BCF), elongation (ELON), and area were 50.29 ? 29.88 mL, 210.64 ? 90.98 x 106/mL, 88.68 ? 10.8 %, 36.64 ? 15.36 %, 63.23 ? 15.98 mm/s, 31.02 ? 9.39 mm/s, 47.53 ? 14.32 mm/s, 65.24 ? 5.72 %, 48.8 ? 8.34 %, 2.68 ? 0.41 mm, 7.29 ? 0.84 Hz, 36.2 ? 3.59 %, 15.49 ? 1.54 mm2, respectively. There were significant differences among stallions in all evaluated parameters of sperm (P &lt; .05). There were significant positive correlations between area and velocity parameters (VSL, VAP, and wobble coefficient [WOB]). The elongation positively correlated with MOT. More elongated sperms demonstrated a lower frequency of curvilinear path intersections with the average path.

Název v anglickém jazyce

Computer-Assisted Sperm Analysis of Head Morphometry and Kinematic Parameters in Warmblood Stallions Spermatozoa

Popis výsledku anglicky

The present study was conducted to determine the seminal characteristics of warmblooded stallions and to assess a relationship between motility parameters of spermatozoa and sperm head dimensions using computer-assisted sperm analysis. In total, 35 ejaculates were collected during the breeding season 2016 from 10 clinically healthy and fertile warmblooded stallions (age 3?22 years). The volume of ejaculate (mL), sperm concentration (M/mL), motility of sperm with kinematic parameters, and sperm morphometry were evaluated. The motility characteristics, sperm concentration, and morphometric parameters of sperm were determined objectively using Sperm Class Analyzer (SCA, MICROPTIC SL). The overall mean ? standard deviation values for volume of ejaculate, concentration, total motility (MOT), progressive motility (PMOT), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), straightness (STR), linearity (LIN), amplitude of lateral head displacement (ALH), beat-cross frequency (BCF), elongation (ELON), and area were 50.29 ? 29.88 mL, 210.64 ? 90.98 x 106/mL, 88.68 ? 10.8 %, 36.64 ? 15.36 %, 63.23 ? 15.98 mm/s, 31.02 ? 9.39 mm/s, 47.53 ? 14.32 mm/s, 65.24 ? 5.72 %, 48.8 ? 8.34 %, 2.68 ? 0.41 mm, 7.29 ? 0.84 Hz, 36.2 ? 3.59 %, 15.49 ? 1.54 mm2, respectively. There were significant differences among stallions in all evaluated parameters of sperm (P &lt; .05). There were significant positive correlations between area and velocity parameters (VSL, VAP, and wobble coefficient [WOB]). The elongation positively correlated with MOT. More elongated sperms demonstrated a lower frequency of curvilinear path intersections with the average path.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Equine Veterinary Science

ISSN

0737-0806

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

říjen

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

8-17

Kód UT WoS článku

000412259900002

EID výsledku v databázi Scopus

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