Chemically defined caseinate extenders and their effect on the cooled semen collected from stallions with long sexual rest

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43902759" target="_blank" >RIV/60076658:12220/21:43902759 - isvavai.cz</a>

Výsledek na webu

<a href="https://jcea.agr.hr/articles/772903_Chemically_defined_caseinate_extenders_and_their_effect_on_the_cooled_semen_collected_from_stallions_with_long_sexual_rest_en.pdf" target="_blank" >https://jcea.agr.hr/articles/772903_Chemically_defined_caseinate_extenders_and_their_effect_on_the_cooled_semen_collected_from_stallions_with_long_sexual_rest_en.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5513/JCEA01/22.2.2904" target="_blank" >10.5513/JCEA01/22.2.2904</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chemically defined caseinate extenders and their effect on the cooled semen collected from stallions with long sexual rest

Popis výsledku v původním jazyce

The ability of three milk-based extenders to preserve the motility and viability of cooled shipped semen produced by stallions after a long sexual rest was evaluated. In total, 21 ejaculates from 11 stallions were collected after 5-6 months of sexual inactivity and diluted with Kenney, INRA 96, and EquiPlus. Seven parameters were evaluated - spermatozoa viability (VIT, %), total motility (MOT, %), progressive motility (PMOT, %), number of progressive spermatozoa per ml (prog M/ml, million/ml), average path velocity (VAP, µm/s), curvilinear velocity (VLC, µm/s) and straight-line velocity of spermatozoa (VSL, µm/s). These parameters were determined by CASA 2 hours after semen processing and then in 24, 48 and 72-hour interval of storage at 5 °C. The efect of storage time was highly significant (P&lt;0.001) for all evaluated parameters. The effect of the extender was significant (P&lt;0.05) for VIT, MOT, PMOT, VCL, VSL and VAP. The ejaculates extended with INRA 96 reached significantly (P&lt;0.01) higher values for all evaluated parameters compared to Kenney extender. The INRA 96 extender significantly increased the values of PMOT, VCL and VAP compared to EquiPlus (P&lt;0.05). In all observed extenders, MOT and VIT decreased linearly whereas PMOT decreased exponentially. The fastest decrease of PMOT was observed within 24 hours of storage. The results obtained from using INRA 96 showed that quality of cooled semen for stallions collected after a long period of sexual inactivity can be sufficiently maintained during at least 24 hours of storage.

Název v anglickém jazyce

Chemically defined caseinate extenders and their effect on the cooled semen collected from stallions with long sexual rest

Popis výsledku anglicky

The ability of three milk-based extenders to preserve the motility and viability of cooled shipped semen produced by stallions after a long sexual rest was evaluated. In total, 21 ejaculates from 11 stallions were collected after 5-6 months of sexual inactivity and diluted with Kenney, INRA 96, and EquiPlus. Seven parameters were evaluated - spermatozoa viability (VIT, %), total motility (MOT, %), progressive motility (PMOT, %), number of progressive spermatozoa per ml (prog M/ml, million/ml), average path velocity (VAP, µm/s), curvilinear velocity (VLC, µm/s) and straight-line velocity of spermatozoa (VSL, µm/s). These parameters were determined by CASA 2 hours after semen processing and then in 24, 48 and 72-hour interval of storage at 5 °C. The efect of storage time was highly significant (P&lt;0.001) for all evaluated parameters. The effect of the extender was significant (P&lt;0.05) for VIT, MOT, PMOT, VCL, VSL and VAP. The ejaculates extended with INRA 96 reached significantly (P&lt;0.01) higher values for all evaluated parameters compared to Kenney extender. The INRA 96 extender significantly increased the values of PMOT, VCL and VAP compared to EquiPlus (P&lt;0.05). In all observed extenders, MOT and VIT decreased linearly whereas PMOT decreased exponentially. The fastest decrease of PMOT was observed within 24 hours of storage. The results obtained from using INRA 96 showed that quality of cooled semen for stallions collected after a long period of sexual inactivity can be sufficiently maintained during at least 24 hours of storage.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Central European Agriculture

ISSN

1332-9049

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

269-279

Kód UT WoS článku

000672536600004

EID výsledku v databázi Scopus

2-s2.0-85110426253