Genetic characterisation of small ruminant lentiviruses in sheep and goats from the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F18%3A43897743" target="_blank" >RIV/60076658:12220/18:43897743 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2754/avb201887010019" target="_blank" >http://dx.doi.org/10.2754/avb201887010019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201887010019" target="_blank" >10.2754/avb201887010019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genetic characterisation of small ruminant lentiviruses in sheep and goats from the Czech Republic

Popis výsledku v původním jazyce

The aim of this study was to determine the prevalence of small ruminant lentivirus (SRLV) infections on sheep and goat farms which are exempt from state monitoring and carry molecular characterisation of strains circulating amongst these farms without SRLV eradication. A total number of 3,410 blood samples of sheep and goats from 21 herds were collected for the purpose of the project. The detected serological prevalence of maedi visna in sheep was 19.9% (556/2801) and the seroprevalence of caprine arthritis and encephalitis in goats was 14.1% (86/609). All positive animals were tested by the nested polymerase chain reaction (nPCR) method for the presence of provirus in the buffy-coats from EDTA-blood samples. Phylogenetic analysis of 93 SRLV strains identified the genotype in 77 sequences, where 60 of them were genotype A and 17 belonged to genotype B. Whereas all of the genotype B sequences were classified in subtype B2, the genotype A group of isolates showed higher variability and were related to subgenotypes A2 and A3. This study represents the first report of genetic characterisation of SRLV strains circulating in the territory of the Czech Republic.

Název v anglickém jazyce

Genetic characterisation of small ruminant lentiviruses in sheep and goats from the Czech Republic

Popis výsledku anglicky

The aim of this study was to determine the prevalence of small ruminant lentivirus (SRLV) infections on sheep and goat farms which are exempt from state monitoring and carry molecular characterisation of strains circulating amongst these farms without SRLV eradication. A total number of 3,410 blood samples of sheep and goats from 21 herds were collected for the purpose of the project. The detected serological prevalence of maedi visna in sheep was 19.9% (556/2801) and the seroprevalence of caprine arthritis and encephalitis in goats was 14.1% (86/609). All positive animals were tested by the nested polymerase chain reaction (nPCR) method for the presence of provirus in the buffy-coats from EDTA-blood samples. Phylogenetic analysis of 93 SRLV strains identified the genotype in 77 sequences, where 60 of them were genotype A and 17 belonged to genotype B. Whereas all of the genotype B sequences were classified in subtype B2, the genotype A group of isolates showed higher variability and were related to subgenotypes A2 and A3. This study represents the first report of genetic characterisation of SRLV strains circulating in the territory of the Czech Republic.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QJ1610096" target="_blank" >QJ1610096: Program zdravotní kontroly lentivirových infekcí malých přežvýkavců s využitím nových metod časné laboratorní diagnostiky a markerů geneticky podmíněné rezistence k infekci jako selekčního kritéria</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

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Svazek periodika

87

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

19-26

Kód UT WoS článku

000431956300003

EID výsledku v databázi Scopus

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