Cryptosporidium apodemi sp n. and Cryptosporidium ditrichi sp n. (Apicomplexa: Cryptosporidiidae) in Apodemus spp.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F18%3A43898017" target="_blank" >RIV/60076658:12220/18:43898017 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498049

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ejop.2017.12.006" target="_blank" >http://dx.doi.org/10.1016/j.ejop.2017.12.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejop.2017.12.006" target="_blank" >10.1016/j.ejop.2017.12.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryptosporidium apodemi sp n. and Cryptosporidium ditrichi sp n. (Apicomplexa: Cryptosporidiidae) in Apodemus spp.

Popis výsledku v původním jazyce

Faecal samples from striped field mice (n = 72) and yellow-necked mice (n = 246) were screened for Cryptosporidium by microscopy and PCR/sequencing. Phylogenetic analysis of small-subunit rRNA, Cryptosporidium oocyst wall protein and actin gene sequences revealed the presence of C. parvum, C. hominis, C. muris and two new species, C. apodemi and C. ditrichi. Oocysts of C. apodemi are smaller than C. ditrichi and both are experimentally infectious for yellow-necked mice but not for common voles. Additionally, infection by C. ditrichi was established in one of three BALB/c mice. The prepatent period was 7-9 and 5-6 days post infection for C. apodemi and C. ditrichi, respectively. The patent period was greater than 30 days for both species. Infection intensity of C. ditrichi ranged from 4000-50,000 oocyst per gram of faeces and developmental stages of C. ditrichi were detected in the jejunum and ileum. In contrast, neither oocysts nor endogenous developmental stages of C. apodemi were detected in faecal or tissue samples, although C. apodemi DNA was detected in contents from the small and large intestine. Morphological, genetic, and biological data support the establishment of C. apodemi and C. ditrichi as a separate species of the genus Cryptosporidium. (C) 2018 Elsevier GmbH. All rights reserved.

Název v anglickém jazyce

Cryptosporidium apodemi sp n. and Cryptosporidium ditrichi sp n. (Apicomplexa: Cryptosporidiidae) in Apodemus spp.

Popis výsledku anglicky

Faecal samples from striped field mice (n = 72) and yellow-necked mice (n = 246) were screened for Cryptosporidium by microscopy and PCR/sequencing. Phylogenetic analysis of small-subunit rRNA, Cryptosporidium oocyst wall protein and actin gene sequences revealed the presence of C. parvum, C. hominis, C. muris and two new species, C. apodemi and C. ditrichi. Oocysts of C. apodemi are smaller than C. ditrichi and both are experimentally infectious for yellow-necked mice but not for common voles. Additionally, infection by C. ditrichi was established in one of three BALB/c mice. The prepatent period was 7-9 and 5-6 days post infection for C. apodemi and C. ditrichi, respectively. The patent period was greater than 30 days for both species. Infection intensity of C. ditrichi ranged from 4000-50,000 oocyst per gram of faeces and developmental stages of C. ditrichi were detected in the jejunum and ileum. In contrast, neither oocysts nor endogenous developmental stages of C. apodemi were detected in faecal or tissue samples, although C. apodemi DNA was detected in contents from the small and large intestine. Morphological, genetic, and biological data support the establishment of C. apodemi and C. ditrichi as a separate species of the genus Cryptosporidium. (C) 2018 Elsevier GmbH. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Protistology

ISSN

0932-4739

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

duben

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

000432527800001

EID výsledku v databázi Scopus

2-s2.0-85041387447