Evidence of transplacental transmission of Encephalitozoon cuniculi genotype II in murine model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F18%3A43898021" target="_blank" >RIV/60076658:12220/18:43898021 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498717

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.exppara.2018.09.001" target="_blank" >http://dx.doi.org/10.1016/j.exppara.2018.09.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.exppara.2018.09.001" target="_blank" >10.1016/j.exppara.2018.09.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evidence of transplacental transmission of Encephalitozoon cuniculi genotype II in murine model

Popis výsledku v původním jazyce

Microsporidia are obligate intracellurar unicellular parasite of wide range of vertebrates. Although ingestion or inhalation of microsporidian spores is the main route of infection, assumed vertical transmission was described in some mammals. The present study was focused on proof of vertical transmission in mice under experimental conditions. Mice were infected with E. cuniculi genotype II intraperitoneally after mating, or perorally followed by mating in acute or chronic phase of infection. Fetuses were delivered by Caesarean section or mice were kept up to the parturition. Some of cubs were immediately after birth transferred to uninfected surrogate mothers. Group of cubs was immunosuppressed. All cubs were examined using polymerase chain reaction for the presence of Encephalitozoon after birth or in their age of 3 or 6 weeks, respectively. All fetuses delivered by Caesarean section, which were intraperitoneally or perorally infected were negative as well as all neonatal mice and youngsters tested in age of 6 weeks. Only immunosuppressed cubs and cubs of immunodeficient mice in age of 21 days were positive for Encephalitozoon cuniculi genotype II. Present results provided the evidence that transplacental transmission of Encephalitozoon cuniculi in mice occurs, but the mechanism of these transport is still unknown.

Název v anglickém jazyce

Evidence of transplacental transmission of Encephalitozoon cuniculi genotype II in murine model

Popis výsledku anglicky

Microsporidia are obligate intracellurar unicellular parasite of wide range of vertebrates. Although ingestion or inhalation of microsporidian spores is the main route of infection, assumed vertical transmission was described in some mammals. The present study was focused on proof of vertical transmission in mice under experimental conditions. Mice were infected with E. cuniculi genotype II intraperitoneally after mating, or perorally followed by mating in acute or chronic phase of infection. Fetuses were delivered by Caesarean section or mice were kept up to the parturition. Some of cubs were immediately after birth transferred to uninfected surrogate mothers. Group of cubs was immunosuppressed. All cubs were examined using polymerase chain reaction for the presence of Encephalitozoon after birth or in their age of 3 or 6 weeks, respectively. All fetuses delivered by Caesarean section, which were intraperitoneally or perorally infected were negative as well as all neonatal mice and youngsters tested in age of 6 weeks. Only immunosuppressed cubs and cubs of immunodeficient mice in age of 21 days were positive for Encephalitozoon cuniculi genotype II. Present results provided the evidence that transplacental transmission of Encephalitozoon cuniculi in mice occurs, but the mechanism of these transport is still unknown.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/GA17-12871S" target="_blank" >GA17-12871S: Objasnění rozdílné virulence a lékové resistence genotypů Encephalitozoon cuniculi v myším modelu.</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Parasitology

ISSN

0014-4894

e-ISSN

—

Svazek periodika

193

Číslo periodika v rámci svazku

říjen

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

51-57

Kód UT WoS článku

000447232500007

EID výsledku v databázi Scopus

2-s2.0-85053776958