The course of infection of Encephalitozoon cuniculi genotype I in mice possess combination of features reported in genotypes II and III
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43903261" target="_blank" >RIV/60076658:12220/21:43903261 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60076658:12310/21:43903261 RIV/60077344:_____/21:00554927
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0014489421000382?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014489421000382?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.exppara.2021.108101" target="_blank" >10.1016/j.exppara.2021.108101</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The course of infection of Encephalitozoon cuniculi genotype I in mice possess combination of features reported in genotypes II and III
Popis výsledku v původním jazyce
Out of three genotypes of Encephalitozoon cuniculi (I-III) available for experimental studies, E. cuniculi genotype I remains the less characterized. This study describes for the first time individual phases of microsporidiosis caused by E. cuniculi genotype I and efficacy of albendazole treatment in immunocompetent BALB/c and C57Bl/6 mice and immunodeficient SCID, CD4(-/-) and CD8(-/-) mice using molecular detection and quantification methods. We demonstrate asymptomatic infection despite an intense dissemination of microsporidia into most organs within the first weeks post infection, followed by a chronic infection characterized by significant microsporidia persistence in immunocompetent, CD4(-/-) and CD8(-/-) mice and a lethal outcome for SCID mice. Albendazole application led to loss E. cuniculi genotype I infection in immunocompetent mouse strains, decreased spore burden by half in CD4(-/-) and CD8(-/-) mice, and prolongation of survival of SCID mice. These results showed Encephalitozoon cuniculi genotype I infection extend and albendazole sensitivity was comparable to E. cuniculi genotype II, but the infection onset speed and mortality rate was similar to E. cuniculi genotype III. These imply that differences in the course of infection and the response to treatment depend not only on immunological status of the host, but also on the genotype causing the infection.
Název v anglickém jazyce
The course of infection of Encephalitozoon cuniculi genotype I in mice possess combination of features reported in genotypes II and III
Popis výsledku anglicky
Out of three genotypes of Encephalitozoon cuniculi (I-III) available for experimental studies, E. cuniculi genotype I remains the less characterized. This study describes for the first time individual phases of microsporidiosis caused by E. cuniculi genotype I and efficacy of albendazole treatment in immunocompetent BALB/c and C57Bl/6 mice and immunodeficient SCID, CD4(-/-) and CD8(-/-) mice using molecular detection and quantification methods. We demonstrate asymptomatic infection despite an intense dissemination of microsporidia into most organs within the first weeks post infection, followed by a chronic infection characterized by significant microsporidia persistence in immunocompetent, CD4(-/-) and CD8(-/-) mice and a lethal outcome for SCID mice. Albendazole application led to loss E. cuniculi genotype I infection in immunocompetent mouse strains, decreased spore burden by half in CD4(-/-) and CD8(-/-) mice, and prolongation of survival of SCID mice. These results showed Encephalitozoon cuniculi genotype I infection extend and albendazole sensitivity was comparable to E. cuniculi genotype II, but the infection onset speed and mortality rate was similar to E. cuniculi genotype III. These imply that differences in the course of infection and the response to treatment depend not only on immunological status of the host, but also on the genotype causing the infection.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10607 - Virology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Experimental Parasitology
ISSN
0014-4894
e-ISSN
—
Svazek periodika
224
Číslo periodika v rámci svazku
MAY 2021
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
10
Strana od-do
—
Kód UT WoS článku
000642099500006
EID výsledku v databázi Scopus
2-s2.0-85103423580