Mechanisms of different levels of opium composition in selected lineages of opium poppy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43902874" target="_blank" >RIV/60076658:12220/21:43902874 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.scientific-publications.net/en/article/1002150/" target="_blank" >https://www.scientific-publications.net/en/article/1002150/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mechanisms of different levels of opium composition in selected lineages of opium poppy

Popis výsledku v původním jazyce

Opium poppy is cultivated for both alkaloids, that can be used in pharmacology as well as for seed, that is valuable in food production. In both cases, levels of alkaloid production are important, even though desired levels are antagonistic. Using bioinformatical approaches we have found full or partial duplications of genes responsible for different steps in morphine production pathways. We hypothesize, that gene duplications or deletions are responsible for different alkaloid content in different poppy varieties. We have tested this hypothesis by qPCR detection of gene duplications in two contrasting (Buddha – high morphine and Redy – low morphine) varieties of poppy and found partial duplications of SalS, SalR, SalAT, NCS2, CODM genes, and whole 4OMT gene duplication in the Buddha variety. In the future work, we will test this hypothesis on more varieties and confirm our findings by FISH and sequencing.

Název v anglickém jazyce

Mechanisms of different levels of opium composition in selected lineages of opium poppy

Popis výsledku anglicky

Opium poppy is cultivated for both alkaloids, that can be used in pharmacology as well as for seed, that is valuable in food production. In both cases, levels of alkaloid production are important, even though desired levels are antagonistic. Using bioinformatical approaches we have found full or partial duplications of genes responsible for different steps in morphine production pathways. We hypothesize, that gene duplications or deletions are responsible for different alkaloid content in different poppy varieties. We have tested this hypothesis by qPCR detection of gene duplications in two contrasting (Buddha – high morphine and Redy – low morphine) varieties of poppy and found partial duplications of SalS, SalR, SalAT, NCS2, CODM genes, and whole 4OMT gene duplication in the Buddha variety. In the future work, we will test this hypothesis on more varieties and confirm our findings by FISH and sequencing.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QK1810391" target="_blank" >QK1810391: Využití technik genomiky a transkriptomiky k tvorbě genových zdrojů a výchozích materiálů máku se specifickými vlastnostmi</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agriculture &amp; Food

ISSN

1314-8591

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

2021

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

7

Strana od-do

116-122

Kód UT WoS článku

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EID výsledku v databázi Scopus

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