Genetický polymorfismus v rámci Leishmania donovani komplexu: korelace s geografickým původem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F04%3A00005679" target="_blank" >RIV/60076658:12310/04:00005679 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Genetic polymorphism within the Leishmania donovani complex: correlation with geographical origin

Popis výsledku v původním jazyce

Random amplified polymorphic DNA (RAPD) was used to detect intraspecific diversity for the Leishmaniadonovani complex. Fifty-two decameric to 21-meric primers of arbitrary sequence were applied to 15 strains thatbelong to nine zymodemes. Strains belonging to the species L. major and L. tropica were used as outgroups. A total of902 amplicons generated by RAPD were scored. Most primers produced species-specific profiles, only 0.6% ampliconswere shared by all species, while 4.3% amplicons were common for all 15 strains of the L. donovani complex. Wellsupportedtrees have been constructed, which show a rather strong correlation between the genetic polymorphism ofstudied strains and their geographic origin. In all obtained trees, L. infantum was paraphyletic. The RAPD profilessuggest that MON-30 belongs to L. donovani. Moreover, the genetic distance between the L. archibaldi strain and otherleishmanias does not warrant existence of a separate species

Název v anglickém jazyce

Genetic polymorphism within the Leishmania donovani complex: correlation with geographical origin

Popis výsledku anglicky

Random amplified polymorphic DNA (RAPD) was used to detect intraspecific diversity for the Leishmaniadonovani complex. Fifty-two decameric to 21-meric primers of arbitrary sequence were applied to 15 strains thatbelong to nine zymodemes. Strains belonging to the species L. major and L. tropica were used as outgroups. A total of902 amplicons generated by RAPD were scored. Most primers produced species-specific profiles, only 0.6% ampliconswere shared by all species, while 4.3% amplicons were common for all 15 strains of the L. donovani complex. Wellsupportedtrees have been constructed, which show a rather strong correlation between the genetic polymorphism ofstudied strains and their geographic origin. In all obtained trees, L. infantum was paraphyletic. The RAPD profilessuggest that MON-30 belongs to L. donovani. Moreover, the genetic distance between the L. archibaldi strain and otherleishmanias does not warrant existence of a separate species

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

American Journal of Tropical Medecine and Hygiene

ISSN

0002-9637

e-ISSN

—

Svazek periodika

70

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

613-617

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—