Characterization of two closely related alpha-amylase paralogs in the bark beetle, Ips typographus (L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F11%3A43882400" target="_blank" >RIV/60076658:12310/11:43882400 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/arch.20433" target="_blank" >http://dx.doi.org/10.1002/arch.20433</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/arch.20433" target="_blank" >10.1002/arch.20433</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of two closely related alpha-amylase paralogs in the bark beetle, Ips typographus (L.)

Popis výsledku v původním jazyce

Ips typographus (L.), the eight-spined spruce bark beetle, causes severe damage throughout Eurasian spruce forests and suitable nuclear markers are needed in order to study its population structure on a genetic level. Two closely related genes encoding alpha-amylase in I. typographus were characterized and named AmyA and AmyB. Both alpha-amylase paralogs consisted of six exons and five introns. AmyA encodes a polypeptide of 483 amino acids, whereas AmyB has two alternative transcripts encoding polypeptides of 483 and 370 amino acids. The expression levels of both genes were high during larval stage and adulthood. The AmyB transcripts were absent in the pupal stage. A modification of the allozyme staining method allowed us to detect two clusters of bands on the electrophoretic gel that may correspond to the two alpha-amylase genes. There was a correlation between the lack of AmyB expression in pupa and the absence of the fast migrating isozyme cluster at this stage, suggesting that the

Název v anglickém jazyce

Characterization of two closely related alpha-amylase paralogs in the bark beetle, Ips typographus (L.)

Popis výsledku anglicky

Ips typographus (L.), the eight-spined spruce bark beetle, causes severe damage throughout Eurasian spruce forests and suitable nuclear markers are needed in order to study its population structure on a genetic level. Two closely related genes encoding alpha-amylase in I. typographus were characterized and named AmyA and AmyB. Both alpha-amylase paralogs consisted of six exons and five introns. AmyA encodes a polypeptide of 483 amino acids, whereas AmyB has two alternative transcripts encoding polypeptides of 483 and 370 amino acids. The expression levels of both genes were high during larval stage and adulthood. The AmyB transcripts were absent in the pupal stage. A modification of the allozyme staining method allowed us to detect two clusters of bands on the electrophoretic gel that may correspond to the two alpha-amylase genes. There was a correlation between the lack of AmyB expression in pupa and the absence of the fast migrating isozyme cluster at this stage, suggesting that the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Insect Biochemistry and Physiology

ISSN

0739-4462

e-ISSN

—

Svazek periodika

77

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

20

Strana od-do

179-198

Kód UT WoS článku

000295287900002

EID výsledku v databázi Scopus

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