Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895478" target="_blank" >RIV/60076658:12310/17:43895478 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/17:00094556

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/abstract;jsessionid=A9D8323BC59677CE806E681153952972.f02t02" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jeu.12341/abstract;jsessionid=A9D8323BC59677CE806E681153952972.f02t02</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jeu.12341" target="_blank" >10.1111/jeu.12341</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea

Popis výsledku v původním jazyce

The study provides robust genetic evidence that a newly isolated naked ameba with morphological and ultrastructural features indicative of Heterolobosea is a new species. Neovahlkampfia nana n. sp. associates with the yet underrepresented subclade of Tetramitia I. Considerable differences found in 18S rRNA gene sequences of individual molecular clones derived from DNA of five clonal cultures, using a low fidelity DNA polymerase, raised the issue of intragenomic sequence variation, a phenomenon that has not been previously studied in Heterolobosea. However, as proved using a higher fidelity DNA polymerase, the sequence variability observed was introduced by PCR mediated by the low fidelity polymerase and fixed by molecular cloning. This points to the potentially dubious validity of some current nominal species of Heterolobosea that differ from one another in just one or two base positions.

Název v anglickém jazyce

Neovahlkampfia nana n. sp Reinforcing an Underrepresented Subclade of Tetramitia, Heterolobosea

Popis výsledku anglicky

The study provides robust genetic evidence that a newly isolated naked ameba with morphological and ultrastructural features indicative of Heterolobosea is a new species. Neovahlkampfia nana n. sp. associates with the yet underrepresented subclade of Tetramitia I. Considerable differences found in 18S rRNA gene sequences of individual molecular clones derived from DNA of five clonal cultures, using a low fidelity DNA polymerase, raised the issue of intragenomic sequence variation, a phenomenon that has not been previously studied in Heterolobosea. However, as proved using a higher fidelity DNA polymerase, the sequence variability observed was introduced by PCR mediated by the low fidelity polymerase and fixed by molecular cloning. This points to the potentially dubious validity of some current nominal species of Heterolobosea that differ from one another in just one or two base positions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Eukaryotic Microbiology

ISSN

1066-5234

e-ISSN

—

Svazek periodika

64

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

78-87

Kód UT WoS článku

000391930200007

EID výsledku v databázi Scopus

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