Leishmania infections: Molecular targets and diagnosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895727" target="_blank" >RIV/60076658:12310/17:43895727 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/17:00482859 RIV/00216208:11310/17:10370268

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0098299716300450" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0098299716300450</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mam.2016.11.012" target="_blank" >10.1016/j.mam.2016.11.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Leishmania infections: Molecular targets and diagnosis

Popis výsledku v původním jazyce

Progress in the diagnosis of leishmaniases depends on the development of effective methods and the discovery of suitable biomarkers. We propose firstly an update classification of Leishmania species and their synonymies. We demonstrate a global map highlighting the geography of known endemic Leishmania species pathogenic to humans. We summarize a complete list of techniques currently in use and discuss their advantages and limitations. The available data highlights the benefits of molecular markers in terms of their sensitivity and specificity to quantify variation from the subgeneric level to species complexes, (sub) species within complexes, and individual populations and infection foci. Each DNA-based detection method is supplied with a comprehensive description of markers and primers and proposal for a classification based on the role of each target and primer in the detection, identification and quantification of leishmaniasis infection. We outline a genome-wide map of genes informative for diagnosis that have been used for Leishmania genotyping. Furthermore, we propose a classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans. This can be applied to newly discovered species and to hybrid strains originating from inter-species crosses. Developing more effective and sensitive diagnostic methods and biomarkers is vital for enhancing Leishmania infection control programs. (C) 2017 The Authors. Published by Elsevier Ltd.

Název v anglickém jazyce

Leishmania infections: Molecular targets and diagnosis

Popis výsledku anglicky

Progress in the diagnosis of leishmaniases depends on the development of effective methods and the discovery of suitable biomarkers. We propose firstly an update classification of Leishmania species and their synonymies. We demonstrate a global map highlighting the geography of known endemic Leishmania species pathogenic to humans. We summarize a complete list of techniques currently in use and discuss their advantages and limitations. The available data highlights the benefits of molecular markers in terms of their sensitivity and specificity to quantify variation from the subgeneric level to species complexes, (sub) species within complexes, and individual populations and infection foci. Each DNA-based detection method is supplied with a comprehensive description of markers and primers and proposal for a classification based on the role of each target and primer in the detection, identification and quantification of leishmaniasis infection. We outline a genome-wide map of genes informative for diagnosis that have been used for Leishmania genotyping. Furthermore, we propose a classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans. This can be applied to newly discovered species and to hybrid strains originating from inter-species crosses. Developing more effective and sensitive diagnostic methods and biomarkers is vital for enhancing Leishmania infection control programs. (C) 2017 The Authors. Published by Elsevier Ltd.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Aspects of Medicine

ISSN

0098-2997

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

OCT 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

29

Strana od-do

1-29

Kód UT WoS článku

000410631000001

EID výsledku v databázi Scopus

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