Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897122" target="_blank" >RIV/60076658:12310/18:43897122 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498849 RIV/60076658:12520/18:43897122

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0378432018301702" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378432018301702</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2018.03.025" target="_blank" >10.1016/j.anireprosci.2018.03.025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

Popis výsledku v původním jazyce

Fish sperm cryopreservation is a well-established technique allowing for artificial insemination on a commercial scale. The extent of proteome alterations in seminal plasma and sperm due to cryopreservation, however, is not known. This study was conducted to evaluate the effect of cryopreservation on motility variables of sterlet Acipenser ruthenus sperm and to detect the differences in protein profiles of fresh and cryopreserved sterlet sperm and seminal plasma. Fresh sperm had 89 +/- 3% motility and 160 +/- 14 mu m/s curvilinear velocity at 15 s post-activation. The motility rate of cryopreserved sperm (37 +/- 5%) was less at 15 s post-activation. No difference (ANOVA; P &gt; 0.05) in mean curvilinear velocity of fresh and cryopreserved sperm was detected. The protein profiles of seminal plasma and sperm were characterized using comparative proteomics to determine the influence of cryopreservation. Six altered protein spots in seminal plasma and thirteen altered spots in sperm were detected in fresh and thawed sperm. Subsequent protein characterization suggested that the proteins identified were involved in sperm metabolism, cytoskeleton, and stress response. The results broaden the understanding of the effects of cryopreservation and identify the proteins associated with cryo-injury. These data may help to determine the function of altered proteins and provide new insights into improving sperm cryopreservation.

Název v anglickém jazyce

Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome

Popis výsledku anglicky

Fish sperm cryopreservation is a well-established technique allowing for artificial insemination on a commercial scale. The extent of proteome alterations in seminal plasma and sperm due to cryopreservation, however, is not known. This study was conducted to evaluate the effect of cryopreservation on motility variables of sterlet Acipenser ruthenus sperm and to detect the differences in protein profiles of fresh and cryopreserved sterlet sperm and seminal plasma. Fresh sperm had 89 +/- 3% motility and 160 +/- 14 mu m/s curvilinear velocity at 15 s post-activation. The motility rate of cryopreserved sperm (37 +/- 5%) was less at 15 s post-activation. No difference (ANOVA; P &gt; 0.05) in mean curvilinear velocity of fresh and cryopreserved sperm was detected. The protein profiles of seminal plasma and sperm were characterized using comparative proteomics to determine the influence of cryopreservation. Six altered protein spots in seminal plasma and thirteen altered spots in sperm were detected in fresh and thawed sperm. Subsequent protein characterization suggested that the proteins identified were involved in sperm metabolism, cytoskeleton, and stress response. The results broaden the understanding of the effects of cryopreservation and identify the proteins associated with cryo-injury. These data may help to determine the function of altered proteins and provide new insights into improving sperm cryopreservation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

—

Svazek periodika

192

Číslo periodika v rámci svazku

05/2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

280-289

Kód UT WoS článku

000431163900034

EID výsledku v databázi Scopus

2-s2.0-85044531629