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Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897599" target="_blank" >RIV/60076658:12310/18:43897599 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/60077344:_____/18:00499985 RIV/60076658:12520/18:43897599

  • Výsledek na webu

    <a href="https://link.springer.com/article/10.1007/s10695-018-0538-5" target="_blank" >https://link.springer.com/article/10.1007/s10695-018-0538-5</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10695-018-0538-5" target="_blank" >10.1007/s10695-018-0538-5</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

  • Popis výsledku v původním jazyce

    The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100g/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 +/- 4% motility and 160 +/- 2m/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 +/- 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1g/mL of AFPIII (58 +/- 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 +/- 6% live cells, while the cryopreserved sperm only contained 26.6 +/- 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1g/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10g/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.

  • Název v anglickém jazyce

    Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

  • Popis výsledku anglicky

    The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100g/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 +/- 4% motility and 160 +/- 2m/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 +/- 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1g/mL of AFPIII (58 +/- 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 +/- 6% live cells, while the cryopreserved sperm only contained 26.6 +/- 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1g/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10g/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    40103 - Fishery

Návaznosti výsledku

  • Projekt

    Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Ostatní

  • Rok uplatnění

    2018

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Fish Physiology and Biochemistry

  • ISSN

    0920-1742

  • e-ISSN

  • Svazek periodika

    44

  • Číslo periodika v rámci svazku

    6

  • Stát vydavatele periodika

    NL - Nizozemsko

  • Počet stran výsledku

    7

  • Strana od-do

    1527-1533

  • Kód UT WoS článku

    000453883600009

  • EID výsledku v databázi Scopus

    2-s2.0-85050196634