Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897599" target="_blank" >RIV/60076658:12310/18:43897599 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60077344:_____/18:00499985 RIV/60076658:12520/18:43897599
Výsledek na webu
<a href="https://link.springer.com/article/10.1007/s10695-018-0538-5" target="_blank" >https://link.springer.com/article/10.1007/s10695-018-0538-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10695-018-0538-5" target="_blank" >10.1007/s10695-018-0538-5</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation
Popis výsledku v původním jazyce
The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100g/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 +/- 4% motility and 160 +/- 2m/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 +/- 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1g/mL of AFPIII (58 +/- 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 +/- 6% live cells, while the cryopreserved sperm only contained 26.6 +/- 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1g/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10g/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.
Název v anglickém jazyce
Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation
Popis výsledku anglicky
The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100g/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 +/- 4% motility and 160 +/- 2m/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 +/- 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1g/mL of AFPIII (58 +/- 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 +/- 6% live cells, while the cryopreserved sperm only contained 26.6 +/- 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1g/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10g/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40103 - Fishery
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Fish Physiology and Biochemistry
ISSN
0920-1742
e-ISSN
—
Svazek periodika
44
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
1527-1533
Kód UT WoS článku
000453883600009
EID výsledku v databázi Scopus
2-s2.0-85050196634