Cryopreservation of sterlet, Acipenser ruthenus spermatozoa: Evaluation of quality parameters and fine ultrastructure.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00557228" target="_blank" >RIV/60077344:_____/22:00557228 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12520/22:43904456 RIV/60076658:12310/22:43904456

Výsledek na webu

<a href="https://doi.org/10.3389/fmars.2022.783278" target="_blank" >https://doi.org/10.3389/fmars.2022.783278</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmars.2022.783278" target="_blank" >10.3389/fmars.2022.783278</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of sterlet, Acipenser ruthenus spermatozoa: Evaluation of quality parameters and fine ultrastructure.

Popis výsledku v původním jazyce

The present study aimed to evaluate cryo-injury during the cryopreservation process in sterlet (Acipenser ruthenus) sperm, focusing on ultrastructural characteristics. Post-thaw sperm quality parameters, including total motility rate, curvilinear velocity (VCL), linearity (LIN), plasma membrane integrity, antioxidant status, DNA damage, and fine ultrastructure were examined on fresh and cryopreserved sperm with/without addition of a single optimal dose of AFPI (10 mu g/mL). A lower motility rate, VCL and plasma membrane integrity, and increased DNA damage (p < 0.05) were observed in frozen-thawed spermatozoa with/without AFPI compared to fresh spermatozoa. The morphology and ultrastructure of spermatozoa were affected during the cryopreservation process with/without supplementation of AFPI. Morphological abnormalities were observed in mitochondria (49-54%) and flagellum (55-57%) of cryopreserved spermatozoa with/without AFPI compared to fresh spermatozoa. In conclusion, the morphology and ultrastructure of spermatozoa were slightly changed after cryopreservation of sterlet spermatozoa with/without 10 mu g/mL AFPI.

Název v anglickém jazyce

Cryopreservation of sterlet, Acipenser ruthenus spermatozoa: Evaluation of quality parameters and fine ultrastructure.

Popis výsledku anglicky

The present study aimed to evaluate cryo-injury during the cryopreservation process in sterlet (Acipenser ruthenus) sperm, focusing on ultrastructural characteristics. Post-thaw sperm quality parameters, including total motility rate, curvilinear velocity (VCL), linearity (LIN), plasma membrane integrity, antioxidant status, DNA damage, and fine ultrastructure were examined on fresh and cryopreserved sperm with/without addition of a single optimal dose of AFPI (10 mu g/mL). A lower motility rate, VCL and plasma membrane integrity, and increased DNA damage (p < 0.05) were observed in frozen-thawed spermatozoa with/without AFPI compared to fresh spermatozoa. The morphology and ultrastructure of spermatozoa were affected during the cryopreservation process with/without supplementation of AFPI. Morphological abnormalities were observed in mitochondria (49-54%) and flagellum (55-57%) of cryopreserved spermatozoa with/without AFPI compared to fresh spermatozoa. In conclusion, the morphology and ultrastructure of spermatozoa were slightly changed after cryopreservation of sterlet spermatozoa with/without 10 mu g/mL AFPI.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

<a href="/cs/project/EF16_025%2F0007370" target="_blank" >EF16_025/0007370: Reprodukční a genetické postupy pro uchování biodiverzity ryb a akvakulturu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in marine science

ISSN

2296-7745

e-ISSN

2296-7745

Svazek periodika

9

Číslo periodika v rámci svazku

Mar

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

783278

Kód UT WoS článku

000787204700001

EID výsledku v databázi Scopus

2-s2.0-85127565237