Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Luhe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897433" target="_blank" >RIV/60076658:12310/18:43897433 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00497528

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S0166685118301166?token=4FB73829BCF217372E9EF8D2B371813194762437E3B304F61E833F2789125A3608DDF3068938BDC8C7A1BE34B16B4B09" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0166685118301166?token=4FB73829BCF217372E9EF8D2B371813194762437E3B304F61E833F2789125A3608DDF3068938BDC8C7A1BE34B16B4B09</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molbiopara.2018.08.003" target="_blank" >10.1016/j.molbiopara.2018.08.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Luhe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening

Popis výsledku v původním jazyce

The broad fish tapeworm Dibothriocephalus lams is a causative agent of human food-borne disease called diphyllobothriosis. Medical importance, scattered geographical distribution and unknown origin of D. latus in Europe and North America make this species to be an interesting model for population genetics. Microsatellite markers were originally designed by library screening using NGS approach and validated as tools for future studies on population genetics of D. latus. Out of 122 candidates selected after NGS analysis, 110 yielded PCR products of the expected size, and in 78 of them, a declared repetitive motif was confirmed by Sanger sequencing. After the fragment analysis, six loci were proved to be polymorphic and tested for observed (Ho) and expected (He) heterozygosity, and deviations from Hardy-Weinberg equilibrium (HWE). They promise future application in studies on genetic interrelationships, origin and migratory routes of this medically important emerging tapeworm.

Název v anglickém jazyce

Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Luhe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening

Popis výsledku anglicky

The broad fish tapeworm Dibothriocephalus lams is a causative agent of human food-borne disease called diphyllobothriosis. Medical importance, scattered geographical distribution and unknown origin of D. latus in Europe and North America make this species to be an interesting model for population genetics. Microsatellite markers were originally designed by library screening using NGS approach and validated as tools for future studies on population genetics of D. latus. Out of 122 candidates selected after NGS analysis, 110 yielded PCR products of the expected size, and in 78 of them, a declared repetitive motif was confirmed by Sanger sequencing. After the fragment analysis, six loci were proved to be polymorphic and tested for observed (Ho) and expected (He) heterozygosity, and deviations from Hardy-Weinberg equilibrium (HWE). They promise future application in studies on genetic interrelationships, origin and migratory routes of this medically important emerging tapeworm.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Biochemical Parasitology

ISSN

0166-6851

e-ISSN

—

Svazek periodika

225

Číslo periodika v rámci svazku

OCT 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

3

Strana od-do

1-3

Kód UT WoS článku

000450378900001

EID výsledku v databázi Scopus

2-s2.0-85051978241