Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897636" target="_blank" >RIV/60076658:12310/18:43897636 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/18:00498061

Výsledek na webu

<a href="https://rnajournal.cshlp.org/content/24/11/1594" target="_blank" >https://rnajournal.cshlp.org/content/24/11/1594</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1261/rna.066233.118" target="_blank" >10.1261/rna.066233.118</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes

Popis výsledku v původním jazyce

MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA-RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs.

Název v anglickém jazyce

Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes

Popis výsledku anglicky

MRP1/2 is a heteromeric protein complex that functions in the trypanosomatid mitochondrion as part of the RNA editing machinery, which facilitates multiple targeted insertions and deletions of uridines. MRP1/2 was shown to interact with MRB8170, which initiates RNA editing by marking pre-edited mRNAs, while TbRGG2 is required for its efficient progression on pan-edited mRNAs. Both MRP1/2 and TbRGG2 are capable of modulating RNA-RNA interactions in vitro. As determined by using iCLIP and RIP-qPCR, RNAs bound to MRP1/2 are characterized and compared with those associated with MRB8170 and TbRGG2. We provide evidence that MRP1 and MRB8170 have correlated binding and similar RNA crosslinking peak profiles over minimally and never-edited mRNAs. Our results suggest that MRP1 assists MRB8170 in RNA editing on minimally edited mRNAs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RNA-A Publication of the RNA Society

ISSN

1355-8382

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

1594-1606

Kód UT WoS článku

000447362100015

EID výsledku v databázi Scopus

2-s2.0-85055075373