Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897714" target="_blank" >RIV/60076658:12310/18:43897714 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/18:00496756

Výsledek na webu

<a href="https://reader.elsevier.com/reader/sd/pii/S2211926418301516?token=238E5870A1ADC6F45BA321CF475A0AA1C7ABA6E0A00DBA64661AEA7F839A4793C30FB9F5935722E9356D3BF85AB3E932" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S2211926418301516?token=238E5870A1ADC6F45BA321CF475A0AA1C7ABA6E0A00DBA64661AEA7F839A4793C30FB9F5935722E9356D3BF85AB3E932</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2018.09.005" target="_blank" >10.1016/j.algal.2018.09.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation

Popis výsledku v původním jazyce

Parachlorella kessleri (formerly Chlorella kessleri) can accumulate high levels of both starch and lipids that can be used for the production of bioethanol and biodiesel, respectively. We mutagenized P. kessleri by heavy-ion beam irradiation and obtained a biotechnologically promising mutant strain, PK4, for further improvement of lipid productivity in comparison with wild type [1]. PK4 reached two times the optical density and accumulated 2.6 times as much starch in complete urea-phosphate (UP) media compared to Iris-acetate-phosphate (TAP) media under laboratory conditions. Similarly to wild type, PK4 accumulated only negligible amounts of lipids in complete UP media. PK4 accumulated more lipids (WT: 1.17 g L-1, PK4: 1.75 g L-1) and accumulated lipids faster than WT after dilution of the UP media (WT; 0.22 g L-1 day(-1), PK4; 0.43 g L-1 day(-1)). The productivity of PK4 was analyzed in mass culture using a 150-L thin-layer photo bioreactor housed in a temperature and ligh-tuncontrolled glass greenhouse. The PBR consisted of two glass plates (each 6 m long and 1 m wide) at an inclination of 1.6%, arranged in a meandering path and connected with a trough. The culture was diluted four-fold with water on day 7 post-inoculation to ensure prompt nutrient limitation in the UP media. Lipid accumulation in PK4 was significantly induced so that it accumulated 66% of lipid per dry weight. Under 150-L mass cultivation conditions, PK4 showed high biomass productivity (0.82 g L-1 day(-1)) and high lipid productivity (0.59 g L-1 day(-1)). In addition, the whole genome of PK4 was sequenced to clarify the genetic variation upon heavy-ion-beam irradiation by comparison with the reference genome of WT. As a result, genetic differentiation of PK4 was found at three genes encoding endo-1,4-beta-mannanase, an ATP/ADP transporter, and an elicitor response protein.

Název v anglickém jazyce

Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation

Popis výsledku anglicky

Parachlorella kessleri (formerly Chlorella kessleri) can accumulate high levels of both starch and lipids that can be used for the production of bioethanol and biodiesel, respectively. We mutagenized P. kessleri by heavy-ion beam irradiation and obtained a biotechnologically promising mutant strain, PK4, for further improvement of lipid productivity in comparison with wild type [1]. PK4 reached two times the optical density and accumulated 2.6 times as much starch in complete urea-phosphate (UP) media compared to Iris-acetate-phosphate (TAP) media under laboratory conditions. Similarly to wild type, PK4 accumulated only negligible amounts of lipids in complete UP media. PK4 accumulated more lipids (WT: 1.17 g L-1, PK4: 1.75 g L-1) and accumulated lipids faster than WT after dilution of the UP media (WT; 0.22 g L-1 day(-1), PK4; 0.43 g L-1 day(-1)). The productivity of PK4 was analyzed in mass culture using a 150-L thin-layer photo bioreactor housed in a temperature and ligh-tuncontrolled glass greenhouse. The PBR consisted of two glass plates (each 6 m long and 1 m wide) at an inclination of 1.6%, arranged in a meandering path and connected with a trough. The culture was diluted four-fold with water on day 7 post-inoculation to ensure prompt nutrient limitation in the UP media. Lipid accumulation in PK4 was significantly induced so that it accumulated 66% of lipid per dry weight. Under 150-L mass cultivation conditions, PK4 showed high biomass productivity (0.82 g L-1 day(-1)) and high lipid productivity (0.59 g L-1 day(-1)). In addition, the whole genome of PK4 was sequenced to clarify the genetic variation upon heavy-ion-beam irradiation by comparison with the reference genome of WT. As a result, genetic differentiation of PK4 was found at three genes encoding endo-1,4-beta-mannanase, an ATP/ADP transporter, and an elicitor response protein.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Projekt

<a href="/cs/project/GA15-09231S" target="_blank" >GA15-09231S: Růst a buněčný cyklus – mechanismy vzájemné koordinace</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

NOV 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

416-426

Kód UT WoS článku

000447187700045

EID výsledku v databázi Scopus

2-s2.0-85053840822