Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00468874" target="_blank" >RIV/61388971:_____/16:00468874 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60076658:12310/16:43890617
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s10126-015-9664-6" target="_blank" >http://dx.doi.org/10.1007/s10126-015-9664-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10126-015-9664-6" target="_blank" >10.1007/s10126-015-9664-6</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)
Popis výsledku v původním jazyce
The effect of nitrogen and sulphur limitation under high irradiance (PAR) was studied in the green microalga Chlorella fusca (Chlorophyta) in order to follow lipid and/or starch accumulation. Growth, biomass composition and the changes in photosynthetic activity (in vivo chlorophyll a fluorescence) were followed in the trials. The full nutrient culture showed high biomass production and starch accumulation at Day 1, when photosynthetic activity was high. Gradual deprivation (no nutrients added) became evident when photosynthesis was significantly suppressed (Day 3 onwards), which entailed a decrease of maximum relative electron transport rate (rETR(max)) and increase of non-photochemical quenching (NPQ), accompanied by the onset of lipid accumulation and decline in starch content. In N- and S-starved cultures, rETRmax significantly decreased by Day 3, which caused a substantial drop in biomass production, cell number, biovolume and induction of lipid and starch accumulation. High starch content (45-50 % of DW) was found at the initial stage in full nutrient culture and at the stationary phase in nutrient-starved cultures. By the end of the trial, all treatments showed high lipid content (similar to 30 % of DW). The full nutrient culture had higher biomass yield than starved treatments although starch (similar to 0.2 g L-1 day(-1)) and lipid (similar to 0.15 g L-1 day(-1)) productivities were fairly similar in all the cultures. Our results showed that we could enrich biomass of C. fusca (%DW) in lipids using a two-stage strategy (a nutrient replete stage followed by gradual nutrient limitation) while under either procedure, N- or S-starvation, both high lipid and starch contents could be achieved.
Název v anglickém jazyce
Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)
Popis výsledku anglicky
The effect of nitrogen and sulphur limitation under high irradiance (PAR) was studied in the green microalga Chlorella fusca (Chlorophyta) in order to follow lipid and/or starch accumulation. Growth, biomass composition and the changes in photosynthetic activity (in vivo chlorophyll a fluorescence) were followed in the trials. The full nutrient culture showed high biomass production and starch accumulation at Day 1, when photosynthetic activity was high. Gradual deprivation (no nutrients added) became evident when photosynthesis was significantly suppressed (Day 3 onwards), which entailed a decrease of maximum relative electron transport rate (rETR(max)) and increase of non-photochemical quenching (NPQ), accompanied by the onset of lipid accumulation and decline in starch content. In N- and S-starved cultures, rETRmax significantly decreased by Day 3, which caused a substantial drop in biomass production, cell number, biovolume and induction of lipid and starch accumulation. High starch content (45-50 % of DW) was found at the initial stage in full nutrient culture and at the stationary phase in nutrient-starved cultures. By the end of the trial, all treatments showed high lipid content (similar to 30 % of DW). The full nutrient culture had higher biomass yield than starved treatments although starch (similar to 0.2 g L-1 day(-1)) and lipid (similar to 0.15 g L-1 day(-1)) productivities were fairly similar in all the cultures. Our results showed that we could enrich biomass of C. fusca (%DW) in lipids using a two-stage strategy (a nutrient replete stage followed by gradual nutrient limitation) while under either procedure, N- or S-starvation, both high lipid and starch contents could be achieved.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Marine Biotechnology
ISSN
1436-2228
e-ISSN
—
Svazek periodika
18
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
13
Strana od-do
24-36
Kód UT WoS článku
000378136600003
EID výsledku v databázi Scopus
2-s2.0-84953354282