Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903293" target="_blank" >RIV/60076658:12310/21:43903293 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/21:00549673

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs00374-021-01587-9" target="_blank" >https://link.springer.com/article/10.1007%2Fs00374-021-01587-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00374-021-01587-9" target="_blank" >10.1007/s00374-021-01587-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

Popis výsledku v původním jazyce

The product inhibition of the 4-methylumbelliferyl phosphate (MUB-P) decay rate, a measure of potential acid phosphatase activity, has not been considered in most of the published kinetic studies. The aim of this study was to determine the type and strength of the product inhibition in order to better define reaction conditions at which the acid phosphatase activity assay produces unbiased results. The MUB-P decay rate was measured in the forest floor and topsoil organic horizons of two spruce forest catchments at different initial MUB-P and P-PO43- concentrations. The type and strength of the inhibition was analyzed by non-linear regression. We found that MUB-P decay was competitively inhibited by the P-PO43- but also by dissolved organic P. By using estimated kinetic parameters, we calculated an underestimation of potential acid phosphatase activity by up to similar to 20% at concentrations of added MUB-P as high as 300 mu mol g(-1) dry weight and an incubation time one hour due to background inorganic and organic P concentrations in range of units of mu mol per gram of dry soil. We discuss approaches that can be used to minimize the underestimation and show that the previously defined recommendations might not be always sufficient to avoid the bias. Therefore, we recommend to analyze progress curves at a wide range of initial MUB-P concentrations using non-linear methods when the enzyme assay is optimized for a given soil. When changes in inorganic and/or organic P concentrations are expected, we further recommend measuring background inorganic and organic P concentrations.

Název v anglickém jazyce

Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

Popis výsledku anglicky

The product inhibition of the 4-methylumbelliferyl phosphate (MUB-P) decay rate, a measure of potential acid phosphatase activity, has not been considered in most of the published kinetic studies. The aim of this study was to determine the type and strength of the product inhibition in order to better define reaction conditions at which the acid phosphatase activity assay produces unbiased results. The MUB-P decay rate was measured in the forest floor and topsoil organic horizons of two spruce forest catchments at different initial MUB-P and P-PO43- concentrations. The type and strength of the inhibition was analyzed by non-linear regression. We found that MUB-P decay was competitively inhibited by the P-PO43- but also by dissolved organic P. By using estimated kinetic parameters, we calculated an underestimation of potential acid phosphatase activity by up to similar to 20% at concentrations of added MUB-P as high as 300 mu mol g(-1) dry weight and an incubation time one hour due to background inorganic and organic P concentrations in range of units of mu mol per gram of dry soil. We discuss approaches that can be used to minimize the underestimation and show that the previously defined recommendations might not be always sufficient to avoid the bias. Therefore, we recommend to analyze progress curves at a wide range of initial MUB-P concentrations using non-linear methods when the enzyme assay is optimized for a given soil. When changes in inorganic and/or organic P concentrations are expected, we further recommend measuring background inorganic and organic P concentrations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology and Fertility of Soils

ISSN

0178-2762

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

991-1005

Kód UT WoS článku

000687940100001

EID výsledku v databázi Scopus

2-s2.0-85113292047