A new species of cryptic cyanobacteria isolated from the epidermis of a bottlenose dolphin and as a bioaerosol

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903332" target="_blank" >RIV/60076658:12310/21:43903332 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/22:73608905

Výsledek na webu

<a href="https://www.tandfonline.com/doi/full/10.1080/00318884.2021.1968173" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/00318884.2021.1968173</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00318884.2021.1968173" target="_blank" >10.1080/00318884.2021.1968173</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A new species of cryptic cyanobacteria isolated from the epidermis of a bottlenose dolphin and as a bioaerosol

Popis výsledku v původním jazyce

Two cyanobacterial strains, one collected from an epidermal mat present on a dead bottlenose dolphin and the other as a bioaerosol 457 m (1500 ft) above the river, were recently analysed from the St. Johns River, Jacksonville, Florida, USA. Both samples had major phenotypic plasticity which confused morphological identification. Amplicon sequencing of the 16S rRNA gene from the isolates revealed that both samples were closely aligned (branch bootstrap support = 100%) with the recently erected genus Komarekiella. Whole genome sequencing and phylogenetic construction also supported the isolation of a new species of cyanobacteria branching from the Nostoc clade. A total evidence approach of molecular, genetic, and ecological examination of these strains supported the erection of a new species, Komarekiella delphini-convector. A prior study determined that the dolphin with the epidermal mat had low levels of microcystins/nodularins (MCs/NODs) in the hepatic tissue. To investigate whether these toxins originated from the epidermal mat, immunoassay (ELISA) and 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) techniques were conducted on the original mat and subsequent culture samples. The results from both analyses were not conclusive. Genome mining was conducted and revealed diverse biosynthetic capabilities of this species but could not support toxin-producing potential. Further analytical work is required to determine the pathogenic capacity of this epizoic species.

Název v anglickém jazyce

A new species of cryptic cyanobacteria isolated from the epidermis of a bottlenose dolphin and as a bioaerosol

Popis výsledku anglicky

Two cyanobacterial strains, one collected from an epidermal mat present on a dead bottlenose dolphin and the other as a bioaerosol 457 m (1500 ft) above the river, were recently analysed from the St. Johns River, Jacksonville, Florida, USA. Both samples had major phenotypic plasticity which confused morphological identification. Amplicon sequencing of the 16S rRNA gene from the isolates revealed that both samples were closely aligned (branch bootstrap support = 100%) with the recently erected genus Komarekiella. Whole genome sequencing and phylogenetic construction also supported the isolation of a new species of cyanobacteria branching from the Nostoc clade. A total evidence approach of molecular, genetic, and ecological examination of these strains supported the erection of a new species, Komarekiella delphini-convector. A prior study determined that the dolphin with the epidermal mat had low levels of microcystins/nodularins (MCs/NODs) in the hepatic tissue. To investigate whether these toxins originated from the epidermal mat, immunoassay (ELISA) and 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) techniques were conducted on the original mat and subsequent culture samples. The results from both analyses were not conclusive. Genome mining was conducted and revealed diverse biosynthetic capabilities of this species but could not support toxin-producing potential. Further analytical work is required to determine the pathogenic capacity of this epizoic species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Phycologia

ISSN

0031-8884

e-ISSN

—

Svazek periodika

60

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

603-618

Kód UT WoS článku

000701474600001

EID výsledku v databázi Scopus

2-s2.0-85116004986