Dual color triads: synthesis, photophysics and applications in live cell imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903349" target="_blank" >RIV/60076658:12310/21:43903349 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2021/NJ/D1NJ00900A" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2021/NJ/D1NJ00900A</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d1nj00900a" target="_blank" >10.1039/d1nj00900a</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dual color triads: synthesis, photophysics and applications in live cell imaging

Popis výsledku v původním jazyce

Fluorescent labels and probes constitute an important class of organic materials used in the development of sensor systems and imaging platforms for various chemical and molecular biology applications. In the development of fluorescent labels and probes, multichromic environment-sensitive fluorophores are very important. In this work, novel triad systems, including subphthalocyanine, BODIPY and thiophene/thianaphthene units, were prepared and their molecular structures were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and H-1 and C-13 NMR spectroscopy. The optical properties of the subphthalocyanine-BODIPY-thiophene/thianaphthene triads were determined using UV-vis absorption and fluorescence (2D and 3D) spectroscopic methods and quantum chemical calculations. The data were analyzed by comparing the results of the final properties to those of the BODIPY- and subphthalocyanine-alone units. The compounds were also evaluated in live cell imaging using a breast cancer cell line. The novel subphthalocyanine-BODIPY-thiophene/thianaphthene triads displayed dual color emission, which is critically important for the development of novel systems particularly in biomedical applications such as bioimaging and cellular sensor systems.

Název v anglickém jazyce

Dual color triads: synthesis, photophysics and applications in live cell imaging

Popis výsledku anglicky

Fluorescent labels and probes constitute an important class of organic materials used in the development of sensor systems and imaging platforms for various chemical and molecular biology applications. In the development of fluorescent labels and probes, multichromic environment-sensitive fluorophores are very important. In this work, novel triad systems, including subphthalocyanine, BODIPY and thiophene/thianaphthene units, were prepared and their molecular structures were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and H-1 and C-13 NMR spectroscopy. The optical properties of the subphthalocyanine-BODIPY-thiophene/thianaphthene triads were determined using UV-vis absorption and fluorescence (2D and 3D) spectroscopic methods and quantum chemical calculations. The data were analyzed by comparing the results of the final properties to those of the BODIPY- and subphthalocyanine-alone units. The compounds were also evaluated in live cell imaging using a breast cancer cell line. The novel subphthalocyanine-BODIPY-thiophene/thianaphthene triads displayed dual color emission, which is critically important for the development of novel systems particularly in biomedical applications such as bioimaging and cellular sensor systems.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Journal of Chemistry

ISSN

1144-0546

e-ISSN

—

Svazek periodika

45

Číslo periodika v rámci svazku

22

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

9984-9994

Kód UT WoS článku

000651857000001

EID výsledku v databázi Scopus

2-s2.0-85107631772