The effect of preservation on biomass and length estimates and its variation within and between two mayfly species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F23%3A43906461" target="_blank" >RIV/60076658:12310/23:43906461 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10201-023-00715-8" target="_blank" >https://link.springer.com/article/10.1007/s10201-023-00715-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10201-023-00715-8" target="_blank" >10.1007/s10201-023-00715-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of preservation on biomass and length estimates and its variation within and between two mayfly species

Popis výsledku v původním jazyce

Biomass estimation offers insight into aquatic ecosystem processes and represents a powerful tool for measuring biomass in communities. However, the accuracy of this estimate depends considerably on the sample preservation method and biological traits of species. We quantified the effect of commonly used preservatives (70% ethanol, 4% formaldehyde, freezing), preservation time and initial body length on the estimation bias of body length, wet mass and dry mass in two mayfly species with dorsoventrally flattened (Rhithrogena carpatoalpina) and cylindrically elongated (Habroleptoides confusa) bodies. Freezing caused the largest relative bias in the length estimates (up to 18%). Both ethanol and freezing led to significant underestimation of wet mass of preserved animals (up to 26%). The most severe biases were observed in dry weights, where all preservation methods reduced body mass by up to 89%. Importantly, initial body mass influenced the bias caused by preservation, and the larger animals were less affected by mass losses. In contrast, larger individuals were subject to greater length reduction. Additionally, the length-mass relationship for R. carpatoalpina, based on fresh mass of living animals, was significantly different from relationships based on preserved animals. Overall, formaldehyde caused the least biased estimates of length and biomass in both examined species. This case study indicates that direct measurements of unpreserved individuals or length-mass equations based on fresh material are likely the best ways to avoid severe underestimation of biomass in aquatic insects. However, more studies covering a broader range of taxa are needed to reach general conclusions.

Název v anglickém jazyce

The effect of preservation on biomass and length estimates and its variation within and between two mayfly species

Popis výsledku anglicky

Biomass estimation offers insight into aquatic ecosystem processes and represents a powerful tool for measuring biomass in communities. However, the accuracy of this estimate depends considerably on the sample preservation method and biological traits of species. We quantified the effect of commonly used preservatives (70% ethanol, 4% formaldehyde, freezing), preservation time and initial body length on the estimation bias of body length, wet mass and dry mass in two mayfly species with dorsoventrally flattened (Rhithrogena carpatoalpina) and cylindrically elongated (Habroleptoides confusa) bodies. Freezing caused the largest relative bias in the length estimates (up to 18%). Both ethanol and freezing led to significant underestimation of wet mass of preserved animals (up to 26%). The most severe biases were observed in dry weights, where all preservation methods reduced body mass by up to 89%. Importantly, initial body mass influenced the bias caused by preservation, and the larger animals were less affected by mass losses. In contrast, larger individuals were subject to greater length reduction. Additionally, the length-mass relationship for R. carpatoalpina, based on fresh mass of living animals, was significantly different from relationships based on preserved animals. Overall, formaldehyde caused the least biased estimates of length and biomass in both examined species. This case study indicates that direct measurements of unpreserved individuals or length-mass equations based on fresh material are likely the best ways to avoid severe underestimation of biomass in aquatic insects. However, more studies covering a broader range of taxa are needed to reach general conclusions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Limnology

ISSN

1439-8621

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

11

Strana od-do

181-191

Kód UT WoS článku

000963026600001

EID výsledku v databázi Scopus

2-s2.0-85151572608