Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F18%3A00488709" target="_blank" >RIV/68081766:_____/18:00488709 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3354/dao03197" target="_blank" >http://dx.doi.org/10.3354/dao03197</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3354/dao03197" target="_blank" >10.3354/dao03197</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method

Popis výsledku v původním jazyce

The aim of this study was to evaluate the influence of preservation method on the results of parasite community studies. Two host species, European perch Perca fluviatilis and European bitterling Rhodeus amarus, were examined for parasites after having been subjected to 4 different storage treatments: freezing, preservation in 4% formaldehyde or 70% ethanol and transportation of live (fresh) fish as a control. Preservation prior to dissection resulted in a loss of information, leading to incomplete quantitative data (all preservation treatments), qualitative data (ethanol and formaldehyde preservation) and a lowered ability to determine parasites to species level based on morphology compared to dissecting fresh fish. Of the more abundant taxa, only crustaceans and acanthocephalans provided relatively even results between treatments. We conclude that preservation media, such as ethanol or formaldehyde, significantly affects the ability to obtain precise parasite community data, hence, we recommend the use of freshly sacrificed fish for parasite community studies whenever possible. Alternatively, freezing may prove acceptable for evaluating parasite community taxonomic composition.

Název v anglickém jazyce

Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method

Popis výsledku anglicky

The aim of this study was to evaluate the influence of preservation method on the results of parasite community studies. Two host species, European perch Perca fluviatilis and European bitterling Rhodeus amarus, were examined for parasites after having been subjected to 4 different storage treatments: freezing, preservation in 4% formaldehyde or 70% ethanol and transportation of live (fresh) fish as a control. Preservation prior to dissection resulted in a loss of information, leading to incomplete quantitative data (all preservation treatments), qualitative data (ethanol and formaldehyde preservation) and a lowered ability to determine parasites to species level based on morphology compared to dissecting fresh fish. Of the more abundant taxa, only crustaceans and acanthocephalans provided relatively even results between treatments. We conclude that preservation media, such as ethanol or formaldehyde, significantly affects the ability to obtain precise parasite community data, hence, we recommend the use of freshly sacrificed fish for parasite community studies whenever possible. Alternatively, freezing may prove acceptable for evaluating parasite community taxonomic composition.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

<a href="/cs/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - Evropské centrum ichtyoparazitologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diseases of Aquatic Organisms

ISSN

0177-5103

e-ISSN

—

Svazek periodika

127

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

213-224

Kód UT WoS článku

000427021400005

EID výsledku v databázi Scopus

2-s2.0-85043698364