Stick together: isolation and characterization of exopolysaccharides producing psychrotolerant bacteria from Alaskan permafrost soils

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F25%3A43910598" target="_blank" >RIV/60076658:12310/25:43910598 - isvavai.cz</a>

Výsledek na webu

<a href="http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://link.springer.com/content/pdf/10.1007/s00300-025-03435-0.pdf?utm_source=clarivate&getft_integrator=clarivate" target="_blank" >http://chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://link.springer.com/content/pdf/10.1007/s00300-025-03435-0.pdf?utm_source=clarivate&getft_integrator=clarivate</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00300-025-03435-0" target="_blank" >10.1007/s00300-025-03435-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stick together: isolation and characterization of exopolysaccharides producing psychrotolerant bacteria from Alaskan permafrost soils

Popis výsledku v původním jazyce

Bacterial exopolysaccharides (EPS) act as natural biopolymers that bind soil particles together, promoting structural stability and creating protective microenvironments for microbial survival. This study aimed to isolate and characterize potential EPS-producing bacteria from the active layer of two degraded permafrost soils with different hydrological landscapes, and from non-degraded permafrost soil. A total of 64 bacterial isolates were obtained, representing three phyla: Firmicutes, Actinomycetota, and Pseudomonadota. EPS production was assessed by determining the polysaccharide content measured as glucose equivalent, and 26 bacterial isolates were identified as potential EPS producers. Among the bacterial isolates, Curtobacterium oceanosedimentum, Frigoribacterium faeni, Streptomyces strains, Neobacillus bataviensis, and Mesobacillus subterraneus exhibited high polysaccharide concentrations. Uronic acids were present in EPS produced by C. oceanosedimentum and N. bataviensis, while amino sugars were identified in EPS from isolates of Bacillus, Streptomyces, Luteimonas, and Phyllobacterium. Based on 16S rRNA gene sequence similarities, the relative proportion of taxa associated with EPS-producing bacterial isolates such as Bacillus, Peribacillus, and Streptomyces was higher in the dry site, while Neobacillus, Pseudarthrobacter, and Microbacterium were more abundant in the wet and intact sites. This study suggests that EPS production with diverse carbohydrate compositions primarily promotes structural stability in degrading permafrost soils by binding soil particles together and forming protective microenvironments. Additionally, EPS may contribute to nutrient retention and microbial protection under fluctuating environmental conditions, complementing their primary role in soil stability.

Název v anglickém jazyce

Stick together: isolation and characterization of exopolysaccharides producing psychrotolerant bacteria from Alaskan permafrost soils

Popis výsledku anglicky

Bacterial exopolysaccharides (EPS) act as natural biopolymers that bind soil particles together, promoting structural stability and creating protective microenvironments for microbial survival. This study aimed to isolate and characterize potential EPS-producing bacteria from the active layer of two degraded permafrost soils with different hydrological landscapes, and from non-degraded permafrost soil. A total of 64 bacterial isolates were obtained, representing three phyla: Firmicutes, Actinomycetota, and Pseudomonadota. EPS production was assessed by determining the polysaccharide content measured as glucose equivalent, and 26 bacterial isolates were identified as potential EPS producers. Among the bacterial isolates, Curtobacterium oceanosedimentum, Frigoribacterium faeni, Streptomyces strains, Neobacillus bataviensis, and Mesobacillus subterraneus exhibited high polysaccharide concentrations. Uronic acids were present in EPS produced by C. oceanosedimentum and N. bataviensis, while amino sugars were identified in EPS from isolates of Bacillus, Streptomyces, Luteimonas, and Phyllobacterium. Based on 16S rRNA gene sequence similarities, the relative proportion of taxa associated with EPS-producing bacterial isolates such as Bacillus, Peribacillus, and Streptomyces was higher in the dry site, while Neobacillus, Pseudarthrobacter, and Microbacterium were more abundant in the wet and intact sites. This study suggests that EPS production with diverse carbohydrate compositions primarily promotes structural stability in degrading permafrost soils by binding soil particles together and forming protective microenvironments. Additionally, EPS may contribute to nutrient retention and microbial protection under fluctuating environmental conditions, complementing their primary role in soil stability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GC20-21259J" target="_blank" >GC20-21259J: CRYOVULCAN - Kryosoly a citlivost uhlíku k dekompozici - interakce substrátu-mikroorganismů-půdních agregátů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Polar Biology

ISSN

0722-4060

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

nestránkováno

Kód UT WoS článku

001621649200003

EID výsledku v databázi Scopus

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