Isolation and identification of bacteria isolates from arsenic contaminated anthroposoils

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27350%2F14%3A86089567" target="_blank" >RIV/61989100:27350/14:86089567 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.5593/SGEM2014/B51/S20.054" target="_blank" >http://dx.doi.org/10.5593/SGEM2014/B51/S20.054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5593/SGEM2014/B51/S20.054" target="_blank" >10.5593/SGEM2014/B51/S20.054</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and identification of bacteria isolates from arsenic contaminated anthroposoils

Popis výsledku v původním jazyce

This study aims to isolate and identify autochthonous bacteria from arseniccontaminated soils collected in the locality of Zemianske Kostoľany. After the coal ash pond spill in 1965, soils, sediments, and ground water were all significantly contaminated with arsenic exceeding 1500 ppm and with other potentially toxic elements. Firstly, sixty-three morphologically different bacterial isolates were obtained from samples of contaminated substrates. Consequently, identification of 16S rRNA sequences showed identities to genus Pseudomonas, Rhodococcus, Bacillus, Streptomyces and Chryseobacterium. Out of these, Pseudomonas was the genus of the greatest proportion of isolates in the studied ashy-soil substrates (62.5 %). The genus was represented by the species Pseudomonas sp., P. chlororaphis, P. putida, P. baetica and P. reinekei. The results showed that the genus Bacillus had the second highest abundance (20.83 %) and was represented by species B. cereus and B. pumilus. Two isolates of the genus Bacillus were classified as uncultured soil bacterium clone. There were further recorded two isolates of the genus Chryseobacterium. The genus Streptomyces and Rhodococcus were represented by only one species. Last but not least, 20 % of isolates were not identified to their genus. Results of studied bacterial species diversity, which was able to adapt to living in contaminated environment, are the basis for the application of selected indigenous bacterial species in bioleaching process as one of the potential methods for bioremediation of arsenic-contaminated soils.

Název v anglickém jazyce

Isolation and identification of bacteria isolates from arsenic contaminated anthroposoils

Popis výsledku anglicky

This study aims to isolate and identify autochthonous bacteria from arseniccontaminated soils collected in the locality of Zemianske Kostoľany. After the coal ash pond spill in 1965, soils, sediments, and ground water were all significantly contaminated with arsenic exceeding 1500 ppm and with other potentially toxic elements. Firstly, sixty-three morphologically different bacterial isolates were obtained from samples of contaminated substrates. Consequently, identification of 16S rRNA sequences showed identities to genus Pseudomonas, Rhodococcus, Bacillus, Streptomyces and Chryseobacterium. Out of these, Pseudomonas was the genus of the greatest proportion of isolates in the studied ashy-soil substrates (62.5 %). The genus was represented by the species Pseudomonas sp., P. chlororaphis, P. putida, P. baetica and P. reinekei. The results showed that the genus Bacillus had the second highest abundance (20.83 %) and was represented by species B. cereus and B. pumilus. Two isolates of the genus Bacillus were classified as uncultured soil bacterium clone. There were further recorded two isolates of the genus Chryseobacterium. The genus Streptomyces and Rhodococcus were represented by only one species. Last but not least, 20 % of isolates were not identified to their genus. Results of studied bacterial species diversity, which was able to adapt to living in contaminated environment, are the basis for the application of selected indigenous bacterial species in bioleaching process as one of the potential methods for bioremediation of arsenic-contaminated soils.

Druh

D - Stať ve sborníku

CEP obor

DH - Báňský průmysl včetně těžby a zpracování uhlí

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

SGEM 2014 : GeoConference on Nano, Bio and Green-Technologies for a Sustainable Future : 17-26, June, 2014, Albena, Bulgaria : Volume I, Micro and Nano Technologies Advances in Biotechnogy

ISBN

978-619-7105-17-9

ISSN

1314-2704

e-ISSN

—

Počet stran výsledku

6

Strana od-do

399-404

Název nakladatele

STEF92 Technology Ltd.

Místo vydání

Sofia

Místo konání akce

Albena

Datum konání akce

17. 6. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000370816200054