Dynamics of ATP and movement in Eurasian perch (Perca fluviatilis L.) sperm in conditions of decreasing osmolality

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F09%3A00010327" target="_blank" >RIV/60076658:12520/09:00010327 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dynamics of ATP and movement in Eurasian perch (Perca fluviatilis L.) sperm in conditions of decreasing osmolality

Popis výsledku v původním jazyce

Repetitive activation of perch (Perca fluviatilis L.) sperm motility was investigated in this study. The first phase of sperm motility activation was initiated by dilution in a 260 mM glucose solution (75% motility). The second phase of motility was achieved by adding water to previously activated sperm, so that the glucose concentration dropped to 220 mM (24% motility). Finally, the third phase was obtained by further addition of water (down to 90 mM glucose) to the activated sperm suspension (15% motility). Parallel measurements of sperm ATP content were also made. The median value for nonactivated sperm was 43.9 nmol ATP/109 spermatozoa. The ATP concentration decreased significantly from 35 to 7 nmol ATP/109 spermatozoa after successive activationsof motility in the above glucose solutions. Sperm velocity ranged in value from 25 to 330 mm/sec at 10 sec postactivation, from 10 to 290 mm/sec at 30 sec, and from 0 to 200 mm/sec at 45 sec. A model postulating several classes in the pop

Název v anglickém jazyce

Dynamics of ATP and movement in Eurasian perch (Perca fluviatilis L.) sperm in conditions of decreasing osmolality

Popis výsledku anglicky

Repetitive activation of perch (Perca fluviatilis L.) sperm motility was investigated in this study. The first phase of sperm motility activation was initiated by dilution in a 260 mM glucose solution (75% motility). The second phase of motility was achieved by adding water to previously activated sperm, so that the glucose concentration dropped to 220 mM (24% motility). Finally, the third phase was obtained by further addition of water (down to 90 mM glucose) to the activated sperm suspension (15% motility). Parallel measurements of sperm ATP content were also made. The median value for nonactivated sperm was 43.9 nmol ATP/109 spermatozoa. The ATP concentration decreased significantly from 35 to 7 nmol ATP/109 spermatozoa after successive activationsof motility in the above glucose solutions. Sperm velocity ranged in value from 25 to 330 mm/sec at 10 sec postactivation, from 10 to 290 mm/sec at 30 sec, and from 0 to 200 mm/sec at 45 sec. A model postulating several classes in the pop

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Theriogenology

ISSN

0093-691X

e-ISSN

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Svazek periodika

72

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000269769500013

EID výsledku v databázi Scopus

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