Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43898754" target="_blank" >RIV/60076658:12520/18:43898754 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0044848617325620" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0044848617325620</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2018.07.067" target="_blank" >10.1016/j.aquaculture.2018.07.067</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

Popis výsledku v původním jazyce

High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J software and TEM, respectively. Sperm motility was assessed using Image J software combined with a computer assisted sperm analysis plugin adapted for assessing P. margaritifera spermatozoa. Other sperm quality parameters evaluated included O-2 consumption, ATP content, and creatine kinase activity. Frozen/thawed spermatozoa exhibited damage to the head but retained a compact spherical shape. Sperm motility indicators showed a significant decrease in quality resulting from the freeze/thaw process. The percent of motile cells was 54% compared to 84% in fresh sperm, O-2 consumption was 4.8 compared to 44 nanomol min(-1), ATP content was 0.72 nmol/10(9) spermatozoa in the activating medium compared to 4.54 nmol/10(9) spermatozoa, and creatine kinase activity was 9.06 x 10(-5) IU mg(-1) protein compared to 12.5 x 10(-5) IU mg(-1) protein. The cryopreservation protocol allowed obtaining an acceptable motility rate after thawing, confirming the predictive value of sperm motility measurements before cryopreservation in terms of their ability to withstand freezing process.

Název v anglickém jazyce

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

Popis výsledku anglicky

High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J software and TEM, respectively. Sperm motility was assessed using Image J software combined with a computer assisted sperm analysis plugin adapted for assessing P. margaritifera spermatozoa. Other sperm quality parameters evaluated included O-2 consumption, ATP content, and creatine kinase activity. Frozen/thawed spermatozoa exhibited damage to the head but retained a compact spherical shape. Sperm motility indicators showed a significant decrease in quality resulting from the freeze/thaw process. The percent of motile cells was 54% compared to 84% in fresh sperm, O-2 consumption was 4.8 compared to 44 nanomol min(-1), ATP content was 0.72 nmol/10(9) spermatozoa in the activating medium compared to 4.54 nmol/10(9) spermatozoa, and creatine kinase activity was 9.06 x 10(-5) IU mg(-1) protein compared to 12.5 x 10(-5) IU mg(-1) protein. The cryopreservation protocol allowed obtaining an acceptable motility rate after thawing, confirming the predictive value of sperm motility measurements before cryopreservation in terms of their ability to withstand freezing process.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

—

Svazek periodika

497

Číslo periodika v rámci svazku

DEC 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

278-286

Kód UT WoS článku

000442900300035

EID výsledku v databázi Scopus

2-s2.0-85051024839