In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F10%3A00011434" target="_blank" >RIV/60076658:12520/10:00011434 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei)

Popis výsledku v původním jazyce

P>In the present study, the effects of Bisphenol A (BPA) on sperm motility and velocity were investigated in perch (Perca fluviatilis L.). Sperm of five mature males was separately collected in a syringe. The sperm samples were diluted in an immobilizingsolution containing 180 mm NaCl, 2.7 mm KCl, 1.4 mm CaCl2, 2.4 mm NaHCO3, pH 8.0 (340 mOsmol kg-1) at ratio 1 : 50 (sperm : IS). The sperm motility was triggered in an activation solution composed of 50 mm NaCl, 20 mm Tris, pH 8.5 containing different concentrations of BPA (2, 1.5, 1.25, 1.0, 0.5, 0.25, 0.125 and 0.0 mm) at ratio 1 : 50 (sperm : AS). At 15 s post-activation, sperm motility and velocity decreased significantly in activation medium containing 1.5 and 1 mm BPA, respectively. The motilityof sperm was totally inhibited in 2 mm of BPA. Sperm motility duration decreased with increasing BPA concentrations. In the control, 25.3% of spermatozoa were still motile at 90 s post-activation, while only 1.3% was in 0.12 mm. However,

Název v anglickém jazyce

In vitro effects of Bisphenol A on sperm motility characteristics in Perca fluviatilis L. (Percidae; Teleostei)

Popis výsledku anglicky

P>In the present study, the effects of Bisphenol A (BPA) on sperm motility and velocity were investigated in perch (Perca fluviatilis L.). Sperm of five mature males was separately collected in a syringe. The sperm samples were diluted in an immobilizingsolution containing 180 mm NaCl, 2.7 mm KCl, 1.4 mm CaCl2, 2.4 mm NaHCO3, pH 8.0 (340 mOsmol kg-1) at ratio 1 : 50 (sperm : IS). The sperm motility was triggered in an activation solution composed of 50 mm NaCl, 20 mm Tris, pH 8.5 containing different concentrations of BPA (2, 1.5, 1.25, 1.0, 0.5, 0.25, 0.125 and 0.0 mm) at ratio 1 : 50 (sperm : AS). At 15 s post-activation, sperm motility and velocity decreased significantly in activation medium containing 1.5 and 1 mm BPA, respectively. The motilityof sperm was totally inhibited in 2 mm of BPA. Sperm motility duration decreased with increasing BPA concentrations. In the control, 25.3% of spermatozoa were still motile at 90 s post-activation, while only 1.3% was in 0.12 mm. However,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Ichthyology

ISSN

0175-8659

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000281894400013

EID výsledku v databázi Scopus

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