Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004198" target="_blank" >RIV/60076658:_____/02:00004198 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12610/02:00005770

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa.

Popis výsledku v původním jazyce

Motility parameters of paddlefish (Polyodon spathula) spermatozoa, in different environmental conditions were investigated in this study. Paddlefish spermatozoa demonstrated the following characteristics: 100% of the cells were motile 10 s after activation with velocity 130-160 #m.s-1, then after 2 min velocity decreased to 80-130 #m.s-1; the period of motility was up to 9 minutes. Concentrations between 0.5 to 5 mM of KCl prevented activation of spermatozoa. When transferred into a swimming medium (20mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined 5 mM of NaCl or MgCl2) 80-100% cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm motility at 10, 40, 50 and 60 s after activation with stable velocity of sperm around 140 #m.s-1. Very low concentration of 0.125 mM CaCl2 combined with 0.5 mM KCl initiated motility in 20% of sperm while all sperm were activated after 2 minutes with 0.25 mM CaCl2 in similar medium for the full peri

Název v anglickém jazyce

Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa.

Popis výsledku anglicky

Motility parameters of paddlefish (Polyodon spathula) spermatozoa, in different environmental conditions were investigated in this study. Paddlefish spermatozoa demonstrated the following characteristics: 100% of the cells were motile 10 s after activation with velocity 130-160 #m.s-1, then after 2 min velocity decreased to 80-130 #m.s-1; the period of motility was up to 9 minutes. Concentrations between 0.5 to 5 mM of KCl prevented activation of spermatozoa. When transferred into a swimming medium (20mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined 5 mM of NaCl or MgCl2) 80-100% cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm motility at 10, 40, 50 and 60 s after activation with stable velocity of sperm around 140 #m.s-1. Very low concentration of 0.125 mM CaCl2 combined with 0.5 mM KCl initiated motility in 20% of sperm while all sperm were activated after 2 minutes with 0.25 mM CaCl2 in similar medium for the full peri

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction

ISSN

1470-1626

e-ISSN

—

Svazek periodika

neuveden

Číslo periodika v rámci svazku

124

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

713-719

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—