Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F03%3A00004986" target="_blank" >RIV/60076658:12610/03:00004986 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm

Popis výsledku v původním jazyce

Concentrations between 0.5 to 5 mM of KCl in the swimming medium (SM) prevented activation of spermatozoa. When transferred into a SM (SM = 20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined with 5 mM of NaCl or MgCl2) 80-100% cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm at 10 - 60 seconds after activation up to 140 #m.s-1. Very low concentration CaCl2 (0.125 mM) combined with 0.5 mM KCl allowed initiate motility in 20% of sperm while 100 % sperm were activated at 2 minutes with 0.25 mM CaCl2 in SM. We also demonstrate that potassium (5-15 mM) inhibited demembranated sperm. Thus, movement initiation of paddlefish spermatozoa is under antagonistic control of K+ and Ca2+ ions concentration. The osmolality of seminal fluid was ranging 33.0 - 62.7 mOsmol.kg-1. The highest ionic concentration of 11.11 mmol.l-1 in seminal fluid was observed for Na+ ion. Then other concentrations declined on 1.56, 0.52 and 0.36 mmol.l-1 for K

Název v anglickém jazyce

Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm

Popis výsledku anglicky

Concentrations between 0.5 to 5 mM of KCl in the swimming medium (SM) prevented activation of spermatozoa. When transferred into a SM (SM = 20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined with 5 mM of NaCl or MgCl2) 80-100% cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm at 10 - 60 seconds after activation up to 140 #m.s-1. Very low concentration CaCl2 (0.125 mM) combined with 0.5 mM KCl allowed initiate motility in 20% of sperm while 100 % sperm were activated at 2 minutes with 0.25 mM CaCl2 in SM. We also demonstrate that potassium (5-15 mM) inhibited demembranated sperm. Thus, movement initiation of paddlefish spermatozoa is under antagonistic control of K+ and Ca2+ ions concentration. The osmolality of seminal fluid was ranging 33.0 - 62.7 mOsmol.kg-1. The highest ionic concentration of 11.11 mmol.l-1 in seminal fluid was observed for Na+ ion. Then other concentrations declined on 1.56, 0.52 and 0.36 mmol.l-1 for K

Druh

D - Stať ve sborníku

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F03%2F0178" target="_blank" >GA524/03/0178: Detailní studium spermií u modelových druhů chrupavčitých (Chondrostei) a kostnatých (Teleostei) ryb</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of abstracts. Genetics in Aquaculture VIII

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

54

Název nakladatele

International Association for Genetics in Aquaculture

Místo vydání

Mie

Místo konání akce

Mie

Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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