Stage-dependent differences in RNA composition and content affect the outcome of expression profiling in roach (Rutilus rutilus) ovary

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43873477" target="_blank" >RIV/60076658:12520/11:43873477 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cbpa.2011.02.007" target="_blank" >http://dx.doi.org/10.1016/j.cbpa.2011.02.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpa.2011.02.007" target="_blank" >10.1016/j.cbpa.2011.02.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stage-dependent differences in RNA composition and content affect the outcome of expression profiling in roach (Rutilus rutilus) ovary

Popis výsledku v původním jazyce

The influence of changing composition and content of RNA on the results of expression profiling was studied in the ovaries of roach (Rutilus rutilus). The highest total RNA yield was detected in the primary growth (pg) and early cortical alveolus (eca) stages. The total RNA yield gradually decreased through the late cortical alveolus and late vitellogenic stages. In the pg and eca stages, total RNA was characterized by a low percentage of 18S and 28S rRNA and a high content of smaller-sized RNAs, opposite pattern was observed in later stages. The ratio mRNA/total RNA was highest at the pg stage but decreased significantly in later stages. When total RNA was used for RT, the shift in the mRNA/total RNA ratio influenced the results of qPCR of four commonly used reference and two target genes (TG). Therefore, the expression of TG should be related to the mRNA pool using the same input of either mRNA to RT or cDNA to qPCR. Gene expression could be also related to tissue-specific RNA yield

Název v anglickém jazyce

Stage-dependent differences in RNA composition and content affect the outcome of expression profiling in roach (Rutilus rutilus) ovary

Popis výsledku anglicky

The influence of changing composition and content of RNA on the results of expression profiling was studied in the ovaries of roach (Rutilus rutilus). The highest total RNA yield was detected in the primary growth (pg) and early cortical alveolus (eca) stages. The total RNA yield gradually decreased through the late cortical alveolus and late vitellogenic stages. In the pg and eca stages, total RNA was characterized by a low percentage of 18S and 28S rRNA and a high content of smaller-sized RNAs, opposite pattern was observed in later stages. The ratio mRNA/total RNA was highest at the pg stage but decreased significantly in later stages. When total RNA was used for RT, the shift in the mRNA/total RNA ratio influenced the results of qPCR of four commonly used reference and two target genes (TG). Therefore, the expression of TG should be related to the mRNA pool using the same input of either mRNA to RT or cDNA to qPCR. Gene expression could be also related to tissue-specific RNA yield

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: Jihočeské výzkumné centrum akvakultury a biodiverzity hydrocenóz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology

ISSN

1095-6433

e-ISSN

—

Svazek periodika

159

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

141-149

Kód UT WoS článku

000290195500006

EID výsledku v databázi Scopus

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