Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F10%3A10080983" target="_blank" >RIV/00216208:11310/10:10080983 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/10:00346483

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

Popis výsledku v původním jazyce

qPCR tomography was developed to study mRNA localization in complex biological samples that are embedded and cryo-sectioned. After total RNA extraction and reverse transcription, the spatial profiles of mRNAs and other functional RNAs were determined byqPCR. The Xenopus laevis oocyte was selected as model, because of its large size (more than 1 mm) and large amount of total RNA ( 5 mýg). Fifteen sections along the animal-vegetal axis were cut and prepared for quantification of 31 RNA targets using thehigh-throughput real-time RT-PCR (qPCR) BioMark? platform. mRNAs were found to have two localization patterns, animal/central or vegetal. Because of the high resolution in sectioning, it was possible to distinguish two subgroups of the vegetal gene patterns: germ plasm determinant pattern and profile of other vegetal genes.

Název v anglickém jazyce

Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

Popis výsledku anglicky

qPCR tomography was developed to study mRNA localization in complex biological samples that are embedded and cryo-sectioned. After total RNA extraction and reverse transcription, the spatial profiles of mRNAs and other functional RNAs were determined byqPCR. The Xenopus laevis oocyte was selected as model, because of its large size (more than 1 mm) and large amount of total RNA ( 5 mýg). Fifteen sections along the animal-vegetal axis were cut and prepared for quantification of 31 RNA targets using thehigh-throughput real-time RT-PCR (qPCR) BioMark? platform. mRNAs were found to have two localization patterns, animal/central or vegetal. Because of the high resolution in sectioning, it was possible to distinguish two subgroups of the vegetal gene patterns: germ plasm determinant pattern and profile of other vegetal genes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Methods

ISSN

1046-2023

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000277953300013

EID výsledku v databázi Scopus

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