Vnitrobuněčné expresní profily, měřené pomocí real-time PCR tomografie ve vajíčcích Xenopus laevis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00094683" target="_blank" >RIV/68378050:_____/08:00094683 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/08:1892

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte

Popis výsledku v původním jazyce

Real-time tomography is a novel quantitative method for measuring localized RNA-expression profiles within single cells. We show its usefulness by dissecting an X. laevis oocyte to slices along its animal?vegetal axis, extracting RNA and measuring levelsof 18 selected mRNAs by real-time RT-PCR. This identified 2 classes of mRNA, one preferentially located towards the animal, the other towards the vegetal pole. mRNAs in each group show comparable intracellular gradients, suggesting they are produced bysimilar mechanisms. Polarization is substantial, though not extreme,with ca 5% of vegetal gene mRNA molecules at the animal pole and ca 50% in the far most vegetal section. Most animal pole mRNAs were found in the 2nd section from the animal pole and inthe central section where the nucleus is located. mRNA expression profiles did not change after in vitro fertilization; we conclude that the post-fertilization cortical rotation has no detectable effect on intracellular mRNA gradients.

Název v anglickém jazyce

Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte

Popis výsledku anglicky

Real-time tomography is a novel quantitative method for measuring localized RNA-expression profiles within single cells. We show its usefulness by dissecting an X. laevis oocyte to slices along its animal?vegetal axis, extracting RNA and measuring levelsof 18 selected mRNAs by real-time RT-PCR. This identified 2 classes of mRNA, one preferentially located towards the animal, the other towards the vegetal pole. mRNAs in each group show comparable intracellular gradients, suggesting they are produced bysimilar mechanisms. Polarization is substantial, though not extreme,with ca 5% of vegetal gene mRNA molecules at the animal pole and ca 50% in the far most vegetal section. Most animal pole mRNAs were found in the 2nd section from the animal pole and inthe central section where the nucleus is located. mRNA expression profiles did not change after in vitro fertilization; we conclude that the post-fertilization cortical rotation has no detectable effect on intracellular mRNA gradients.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/KJB500520601" target="_blank" >KJB500520601: Kvantifikace a lokalizace maternálních mRNA v časném vývoji drápatky (Xenopus)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

00025302160

EID výsledku v databázi Scopus

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