Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F11%3A43876864" target="_blank" >RIV/60076658:12520/11:43876864 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cbpa.2011.05.026" target="_blank" >http://dx.doi.org/10.1016/j.cbpa.2011.05.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpa.2011.05.026" target="_blank" >10.1016/j.cbpa.2011.05.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm

Popis výsledku v původním jazyce

The present study shows the roles of osmolality, Ca2+- K+ antagonist and Ca2+ in sperm activation and flagellar beating of sturgeon. Sperm motility was activated at hypoosmolality and suppressed at 175 mOsmol kg?1. Sperm activation was suppressed by 0.35mM K+, but Ca2+ could reverse K+ inhibitory effect at Ca2+: K+ ratio of 0.25. Neither EGTA (a chelator of Ca2+) nor nifedipine (a Ca2+ channel blocker) prevented sperm activation. But, sperm motility and velocity were decreased by EGTA, nifedipine and aninhibitor for Ca2+/calmodulin protein (w-7); suggesting role of Ca2+ signaling after triggering sperm activation through hypoosmolality. Symmetric flagellar beating was turned to asymmetric after activation in w-7; evidence for modulation of Ca2+-binding proteins activity. Sturgeon sperm, similar to salmonids, is immotile in seminal plasma due to high K+ , but the mechanism of sperm activation seems to be closer to freshwater teleosts, where osmolality prohibits sperm activation in semi

Název v anglickém jazyce

Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm

Popis výsledku anglicky

The present study shows the roles of osmolality, Ca2+- K+ antagonist and Ca2+ in sperm activation and flagellar beating of sturgeon. Sperm motility was activated at hypoosmolality and suppressed at 175 mOsmol kg?1. Sperm activation was suppressed by 0.35mM K+, but Ca2+ could reverse K+ inhibitory effect at Ca2+: K+ ratio of 0.25. Neither EGTA (a chelator of Ca2+) nor nifedipine (a Ca2+ channel blocker) prevented sperm activation. But, sperm motility and velocity were decreased by EGTA, nifedipine and aninhibitor for Ca2+/calmodulin protein (w-7); suggesting role of Ca2+ signaling after triggering sperm activation through hypoosmolality. Symmetric flagellar beating was turned to asymmetric after activation in w-7; evidence for modulation of Ca2+-binding proteins activity. Sturgeon sperm, similar to salmonids, is immotile in seminal plasma due to high K+ , but the mechanism of sperm activation seems to be closer to freshwater teleosts, where osmolality prohibits sperm activation in semi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology

ISSN

1095-6433

e-ISSN

—

Svazek periodika

160

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

166-174

Kód UT WoS článku

000293936800009

EID výsledku v databázi Scopus

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