Regulation of spermatozoa motility in response to cations in Russian sturgeon Acipenser gueldenstaedtii

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883413" target="_blank" >RIV/60076658:12520/12:43883413 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.theriogenology.2012.01.025" target="_blank" >http://dx.doi.org/10.1016/j.theriogenology.2012.01.025</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.theriogenology.2012.01.025" target="_blank" >10.1016/j.theriogenology.2012.01.025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Regulation of spermatozoa motility in response to cations in Russian sturgeon Acipenser gueldenstaedtii

Popis výsledku v původním jazyce

The aim of this study was to investigate the response of Russian sturgeon (Acipenser gueldenstaedtii) sperm to external cations (Na+, K+, Ca2+, and Mg2+) and their susceptibility on the induction of motility and swimming behavior. An in vitro spermatozoamotility assay was used by a computer-aided Motion-Analysis system. Sperm motility was inhibited by 60 mm NaCl (similar to 140 mOsm/kg) and 0.7 mm KCl solutions (similar to 21.4 mOsm/kg). The Ca2+ and Mg2(+) ions were not able to inhibit spermatozoa motility. By contrast, Na+ within a limited concentration range (between 45 and 55 mm) was able to reverse the inhibitory effect of K+ at the critical concentration (0.7 mm). Ca2+ and Mg2+ were also able to reverse the K+-mediated spermatozoa motility restriction at concentrations starting at 0.01 and 0.1 mm, respectively. These results provide evidence for the role of K+ in suppressing spermatozoa motility, and suggest that Ca2+, Mg2+, and possibly Na+ trigger motility in Russian sturgeon

Název v anglickém jazyce

Regulation of spermatozoa motility in response to cations in Russian sturgeon Acipenser gueldenstaedtii

Popis výsledku anglicky

The aim of this study was to investigate the response of Russian sturgeon (Acipenser gueldenstaedtii) sperm to external cations (Na+, K+, Ca2+, and Mg2+) and their susceptibility on the induction of motility and swimming behavior. An in vitro spermatozoamotility assay was used by a computer-aided Motion-Analysis system. Sperm motility was inhibited by 60 mm NaCl (similar to 140 mOsm/kg) and 0.7 mm KCl solutions (similar to 21.4 mOsm/kg). The Ca2+ and Mg2(+) ions were not able to inhibit spermatozoa motility. By contrast, Na+ within a limited concentration range (between 45 and 55 mm) was able to reverse the inhibitory effect of K+ at the critical concentration (0.7 mm). Ca2+ and Mg2+ were also able to reverse the K+-mediated spermatozoa motility restriction at concentrations starting at 0.01 and 0.1 mm, respectively. These results provide evidence for the role of K+ in suppressing spermatozoa motility, and suggest that Ca2+, Mg2+, and possibly Na+ trigger motility in Russian sturgeon

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Theriogenology

ISSN

0093-691X

e-ISSN

—

Svazek periodika

78

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

102-109

Kód UT WoS článku

000305310900010

EID výsledku v databázi Scopus

—