Optimizing extenders for short-term storage of Sterlet (Acipenser ruthenus) sperm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908162" target="_blank" >RIV/60076658:12520/24:43908162 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aqrep.2024.102216" target="_blank" >https://doi.org/10.1016/j.aqrep.2024.102216</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2024.102216" target="_blank" >10.1016/j.aqrep.2024.102216</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimizing extenders for short-term storage of Sterlet (Acipenser ruthenus) sperm

Popis výsledku v původním jazyce

Considering endangered fish, storage of sterlet (Acipenser ruthenus) gamete is crucial, and investigating the most suitable extender for short-term storage of sperm is necessary. Therefore, the present study aimed to optimize suitable extenders for short-term storage of sterlet spermatozoa and determine the effects of storage periods and different extenders on spermatozoa motility parameters. The sperm of five mature males with good spermatozoa motility (similar to 80 %) were diluted with 20 different extenders having various Na+ and K+ concentrations and osmolality. Spermatozoa motility performances were assessed using a CASA system within 144 h storage at 0-2 degrees C. Undiluted sperm was used as a control. Seminal plasma Na+, K+, Ca2+ and Cl- concentrations were 17.56 +/- 10.12, 2.48 +/- 1.14, 0.11 +/- 0.06, and 6.20 +/- 3.42 mmol/L with osmolality of 35.60 +/- 20.70 mOsm/kg. A two-way ANOVA showed significant effects of storage time, extender, and their interaction on spermatozoa motility, curvilinear velocity (VCL), and straight-line velocity (VSL) (p&lt;0.001). The model was decomposed into one-way ANOVA to study the effects of storage time and extenders. Excluding 1 h post-storage, spermatozoa motility, VCL, and VSL were lower in the non-diluted sperm than those diluted with E18-E20 at 24 h post-storage (p&lt;0.05). The osmolality of these extenders was respectively 46, 55, and 62 mOsmol/kg and contained 1 mM KCl. Increasing the extender KCl concentrations to &gt;3 mM resulted in lower spermatozoa motility performance. When the effects of storage time were studied, spermatozoa motility, VCL, and VSL in non-diluted sperm and those diluted with E18-E20 were decreased at 24-48 h and 72-96 h post-storage, respectively. This study shows that extenders with higher osmolality (39-62 mOsm/kg) and lower K+ (1 mmol/L) are the most suitable for storing sterlet sperm for a short period.

Název v anglickém jazyce

Optimizing extenders for short-term storage of Sterlet (Acipenser ruthenus) sperm

Popis výsledku anglicky

Considering endangered fish, storage of sterlet (Acipenser ruthenus) gamete is crucial, and investigating the most suitable extender for short-term storage of sperm is necessary. Therefore, the present study aimed to optimize suitable extenders for short-term storage of sterlet spermatozoa and determine the effects of storage periods and different extenders on spermatozoa motility parameters. The sperm of five mature males with good spermatozoa motility (similar to 80 %) were diluted with 20 different extenders having various Na+ and K+ concentrations and osmolality. Spermatozoa motility performances were assessed using a CASA system within 144 h storage at 0-2 degrees C. Undiluted sperm was used as a control. Seminal plasma Na+, K+, Ca2+ and Cl- concentrations were 17.56 +/- 10.12, 2.48 +/- 1.14, 0.11 +/- 0.06, and 6.20 +/- 3.42 mmol/L with osmolality of 35.60 +/- 20.70 mOsm/kg. A two-way ANOVA showed significant effects of storage time, extender, and their interaction on spermatozoa motility, curvilinear velocity (VCL), and straight-line velocity (VSL) (p&lt;0.001). The model was decomposed into one-way ANOVA to study the effects of storage time and extenders. Excluding 1 h post-storage, spermatozoa motility, VCL, and VSL were lower in the non-diluted sperm than those diluted with E18-E20 at 24 h post-storage (p&lt;0.05). The osmolality of these extenders was respectively 46, 55, and 62 mOsmol/kg and contained 1 mM KCl. Increasing the extender KCl concentrations to &gt;3 mM resulted in lower spermatozoa motility performance. When the effects of storage time were studied, spermatozoa motility, VCL, and VSL in non-diluted sperm and those diluted with E18-E20 were decreased at 24-48 h and 72-96 h post-storage, respectively. This study shows that extenders with higher osmolality (39-62 mOsm/kg) and lower K+ (1 mmol/L) are the most suitable for storing sterlet sperm for a short period.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

001263176100001

EID výsledku v databázi Scopus

2-s2.0-85196966205