Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909638" target="_blank" >RIV/60076658:12520/25:43909638 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s10695-024-01413-7" target="_blank" >https://doi.org/10.1007/s10695-024-01413-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-024-01413-7" target="_blank" >10.1007/s10695-024-01413-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions

Popis výsledku v původním jazyce

Short-term storage and management of sperm in vitro is an easy and economical process in which suitable extenders can be utilized to extend the storage period and prevent sperm function impairment. Therefore, the current study aimed to evaluate the effect of suitable extenders during the short-term storage of sterlet sperm and determine their fertilizing capacity and hatching success. Three extenders containing a composition of 16, 20, and 24 mM NaCl, 1 mM KCl, 0.1 mM CaCl2, 10 mM Tris, pH 8.0 with osmolarity of 46, 55, and 62 mOsm/kg, were used to dilute the sperm of four sexually mature sterlet males (n = 4). Using a CASA system, the motility and velocity of undiluted and diluted sperm with extenders (E1 - E3) were assessed over 6 days at 0-2 degrees C. The short-term stored diluted sperm was then used in the fertilization and hatching assay, and undiluted fresh and stored sperm was used as a control. A two-way factorial analysis of variance (ANOVA) model confirmed significant effects on sperm motility, curvilinear velocity (VCL), and straight-line velocity (VSL) (P &lt; 0.001), as well as their interaction with the extender. The model was decomposed into a one-way ANOVA to examine the impacts of extenders and storage time. With increasing storage periods, the sperm motility and velocity gradually decreased for diluted sperm with three extenders (E1-E3) but sharply decreased for undiluted sperm (Control). The motility of undiluted sperm was found 3.77 +/- 4.09% at 4 days, whereas sperm diluted with extenders showed 57.57 +/- 12.33% (E1), 64.34 +/- 11.86% (E2), and 61.40 +/- 12.41% (E3) motility at 6 days. This study explored extenders optimized with higher osmolarity (39-62 mOsm/kg) and lower K+ (1 mmol/L) as the most suitable medium for storing sterlet sperm for 6 days. After 6 days post storage, sperm diluted with extenders E1-E3 achieved a fertilization rate of 31.29 +/- 14.2%, 31.66 +/- 8.84%, and 30.67 +/- 10.02%, respectively, and hatching success of 29.58 +/- 13.4%, 30.50 +/- 7.89%, and 27.95 +/- 9.62%, respectively with freshly ovulated eggs.

Název v anglickém jazyce

Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions

Popis výsledku anglicky

Short-term storage and management of sperm in vitro is an easy and economical process in which suitable extenders can be utilized to extend the storage period and prevent sperm function impairment. Therefore, the current study aimed to evaluate the effect of suitable extenders during the short-term storage of sterlet sperm and determine their fertilizing capacity and hatching success. Three extenders containing a composition of 16, 20, and 24 mM NaCl, 1 mM KCl, 0.1 mM CaCl2, 10 mM Tris, pH 8.0 with osmolarity of 46, 55, and 62 mOsm/kg, were used to dilute the sperm of four sexually mature sterlet males (n = 4). Using a CASA system, the motility and velocity of undiluted and diluted sperm with extenders (E1 - E3) were assessed over 6 days at 0-2 degrees C. The short-term stored diluted sperm was then used in the fertilization and hatching assay, and undiluted fresh and stored sperm was used as a control. A two-way factorial analysis of variance (ANOVA) model confirmed significant effects on sperm motility, curvilinear velocity (VCL), and straight-line velocity (VSL) (P &lt; 0.001), as well as their interaction with the extender. The model was decomposed into a one-way ANOVA to examine the impacts of extenders and storage time. With increasing storage periods, the sperm motility and velocity gradually decreased for diluted sperm with three extenders (E1-E3) but sharply decreased for undiluted sperm (Control). The motility of undiluted sperm was found 3.77 +/- 4.09% at 4 days, whereas sperm diluted with extenders showed 57.57 +/- 12.33% (E1), 64.34 +/- 11.86% (E2), and 61.40 +/- 12.41% (E3) motility at 6 days. This study explored extenders optimized with higher osmolarity (39-62 mOsm/kg) and lower K+ (1 mmol/L) as the most suitable medium for storing sterlet sperm for 6 days. After 6 days post storage, sperm diluted with extenders E1-E3 achieved a fertilization rate of 31.29 +/- 14.2%, 31.66 +/- 8.84%, and 30.67 +/- 10.02%, respectively, and hatching success of 29.58 +/- 13.4%, 30.50 +/- 7.89%, and 27.95 +/- 9.62%, respectively with freshly ovulated eggs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

1573-5168

Svazek periodika

51

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

nestránkováno

Kód UT WoS článku

001369106400002

EID výsledku v databázi Scopus

2-s2.0-85211137622