Optimization of short-term storage of pikeperch semen: an applicable approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902525" target="_blank" >RIV/60076658:12520/21:43902525 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.animal.2020.100001" target="_blank" >https://doi.org/10.1016/j.animal.2020.100001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.animal.2020.100001" target="_blank" >10.1016/j.animal.2020.100001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of short-term storage of pikeperch semen: an applicable approach

Popis výsledku v původním jazyce

Contamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at 4 degrees C using two immobilizing media (IM): (a) IM1, 180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2 center dot 2H(2)O and 2.38 mM NaHCO3, 343 mOsm/kg; and (b) IM2, 200 mM NaCl, 2.68 mM Ka, 1.36 mM CaCl2 center dot 2H(2)O and 2.38 mM NaHCO3, 381 mOsm/kg. Undiluted sperm was used as the control. At 6 h poststorage, there were no substantial changes in spermatozoa motility and velocity at 30 s postactivation in all groups. Over 48 h of storage, the highest spermatozoa motility and velocity were obtained in sperm diluted in IM2 compared to the other groups. IM2 could maintain a significantly higher ATP content of diluted sperm than IM1 and undiluted treatment fort days. Similarly, the highest values of eyeing and hatching rates were observed in sperm diluted in IM2 compared to sperm in the other studied groups. It can be concluded that the obtained result is a novel and applicable approach to maintain semen quality of pikeperch during short-term storage, suggesting IM2 as a promising medium for short-term storage. The present study also opens possibilities for ensuring a reliable source of semen as a convenient approach for increasing genetic diversity in hatcheries. (C) 2020 Published by Elsevier Inc. on behalf of The Animal Consortium.

Název v anglickém jazyce

Optimization of short-term storage of pikeperch semen: an applicable approach

Popis výsledku anglicky

Contamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at 4 degrees C using two immobilizing media (IM): (a) IM1, 180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2 center dot 2H(2)O and 2.38 mM NaHCO3, 343 mOsm/kg; and (b) IM2, 200 mM NaCl, 2.68 mM Ka, 1.36 mM CaCl2 center dot 2H(2)O and 2.38 mM NaHCO3, 381 mOsm/kg. Undiluted sperm was used as the control. At 6 h poststorage, there were no substantial changes in spermatozoa motility and velocity at 30 s postactivation in all groups. Over 48 h of storage, the highest spermatozoa motility and velocity were obtained in sperm diluted in IM2 compared to the other groups. IM2 could maintain a significantly higher ATP content of diluted sperm than IM1 and undiluted treatment fort days. Similarly, the highest values of eyeing and hatching rates were observed in sperm diluted in IM2 compared to sperm in the other studied groups. It can be concluded that the obtained result is a novel and applicable approach to maintain semen quality of pikeperch during short-term storage, suggesting IM2 as a promising medium for short-term storage. The present study also opens possibilities for ensuring a reliable source of semen as a convenient approach for increasing genetic diversity in hatcheries. (C) 2020 Published by Elsevier Inc. on behalf of The Animal Consortium.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal

ISSN

1751-7311

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000626637900001

EID výsledku v databázi Scopus

2-s2.0-85100670584