Spermatozoa Motility and Variation in the Seminal Plasma Proteome of Eurasian Perch (Perca fluviatilis) During the Reproductive Season

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F12%3A43883460" target="_blank" >RIV/60076658:12520/12:43883460 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/mrd.22126" target="_blank" >http://dx.doi.org/10.1002/mrd.22126</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mrd.22126" target="_blank" >10.1002/mrd.22126</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Spermatozoa Motility and Variation in the Seminal Plasma Proteome of Eurasian Perch (Perca fluviatilis) During the Reproductive Season

Popis výsledku v původním jazyce

This study evaluated physiological and functional sperm parameters and the seminal plasma proteome of Eurasian perch (Perca fluviatilis) over the course of their reproductive season. Spermatozoa velocity (169.56+/-6.53 to 158.5+/-7.4 mmsec-1), percent motility (95.89+/-4.28% to 89.55+/-4.5%), and osmolality of seminal plasma (290+/-5 to 297+/-12 mOsmol kg-1) remained stable throughout the reproductive season. Milt volume and protein concentration of seminal plasma gradually increased and reached the highest values late in the reproductive period. Spermatozoa concentration peaked in the mid-reproductive season (66.90+/-13x109 spermatozoa ml-1) and decreased towards the end (54+/-10x 109 spermatozoa ml -1). A proteomic analysis of seminal plasma using twodimensional polyacrylamide gel electrophoresis revealed 10 protein spots significantly altered over the course of the reproductive season. Subsequent protein characterization suggested that time in the reproductive season predominantly a

Název v anglickém jazyce

Spermatozoa Motility and Variation in the Seminal Plasma Proteome of Eurasian Perch (Perca fluviatilis) During the Reproductive Season

Popis výsledku anglicky

This study evaluated physiological and functional sperm parameters and the seminal plasma proteome of Eurasian perch (Perca fluviatilis) over the course of their reproductive season. Spermatozoa velocity (169.56+/-6.53 to 158.5+/-7.4 mmsec-1), percent motility (95.89+/-4.28% to 89.55+/-4.5%), and osmolality of seminal plasma (290+/-5 to 297+/-12 mOsmol kg-1) remained stable throughout the reproductive season. Milt volume and protein concentration of seminal plasma gradually increased and reached the highest values late in the reproductive period. Spermatozoa concentration peaked in the mid-reproductive season (66.90+/-13x109 spermatozoa ml-1) and decreased towards the end (54+/-10x 109 spermatozoa ml -1). A proteomic analysis of seminal plasma using twodimensional polyacrylamide gel electrophoresis revealed 10 protein spots significantly altered over the course of the reproductive season. Subsequent protein characterization suggested that time in the reproductive season predominantly a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Reproduction and Development

ISSN

1040-452X

e-ISSN

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Svazek periodika

79

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

879-887

Kód UT WoS článku

000312308500009

EID výsledku v databázi Scopus

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