Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00472830" target="_blank" >RIV/60077344:_____/16:00472830 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/16:43890539 RIV/60076658:12520/16:43890539

Výsledek na webu

<a href="http://dx.doi.org/10.1002/mrd.22737" target="_blank" >http://dx.doi.org/10.1002/mrd.22737</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mrd.22737" target="_blank" >10.1002/mrd.22737</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Popis výsledku v původním jazyce

The purpose of the study was to analyze seminal plasma composition, sperm production, and sperm motility over the course of the spawning season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss). The highest mean percent of motile sperm (carp, 98.3 +/- 1.6%; rainbow trout, 92.8 +/- 5.6%) and highest spermatozoon velocity (carp, 286.3 +/- 7.8msec(-1); rainbow trout, 245.3 +/- 8.3msec(-1)) were observed in the middle phase of the spawning period, at 5sec post-activation. Sperm volume and concentration increased in the early spawning period, then decreased significantly toward the end of the season in both species. Seminal plasma osmolality was 262-270mOsmolkg(-1) from carp and 232-248mOsmolkg(-1) from rainbow trout, with little variation over the spawning period. Protein concentration in carp seminal plasma was stable throughout the reproductive season, whereas the highest protein level (2.1 +/- 0.3mgmL(-1)) in rainbow trout was observed in the middle phase of the spawning period. Seminal plasma composition in both species was analyzed using two-dimensional polyacrylamide gel electrophoresis combined with mass spectrometry. Thirteen differentially expressed protein spots in carp seminal plasma and sixteen spots in rainbow trout seminal plasma varied over the course of the reproductive season. The unique proteins identified are involved in the regulation of spermatogenesis, sperm motility, maintenance of sperm membrane lipid stability, and antioxidant protection. These results provide a deeper understanding of the role of fish seminal plasma proteins as well as a list of novel markers of sperm quality. Mol. Reprod. Dev. 83: 968-982, 2016 (c) 2016 Wiley Periodicals, Inc.

Název v anglickém jazyce

Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Popis výsledku anglicky

The purpose of the study was to analyze seminal plasma composition, sperm production, and sperm motility over the course of the spawning season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss). The highest mean percent of motile sperm (carp, 98.3 +/- 1.6%; rainbow trout, 92.8 +/- 5.6%) and highest spermatozoon velocity (carp, 286.3 +/- 7.8msec(-1); rainbow trout, 245.3 +/- 8.3msec(-1)) were observed in the middle phase of the spawning period, at 5sec post-activation. Sperm volume and concentration increased in the early spawning period, then decreased significantly toward the end of the season in both species. Seminal plasma osmolality was 262-270mOsmolkg(-1) from carp and 232-248mOsmolkg(-1) from rainbow trout, with little variation over the spawning period. Protein concentration in carp seminal plasma was stable throughout the reproductive season, whereas the highest protein level (2.1 +/- 0.3mgmL(-1)) in rainbow trout was observed in the middle phase of the spawning period. Seminal plasma composition in both species was analyzed using two-dimensional polyacrylamide gel electrophoresis combined with mass spectrometry. Thirteen differentially expressed protein spots in carp seminal plasma and sixteen spots in rainbow trout seminal plasma varied over the course of the reproductive season. The unique proteins identified are involved in the regulation of spermatogenesis, sperm motility, maintenance of sperm membrane lipid stability, and antioxidant protection. These results provide a deeper understanding of the role of fish seminal plasma proteins as well as a list of novel markers of sperm quality. Mol. Reprod. Dev. 83: 968-982, 2016 (c) 2016 Wiley Periodicals, Inc.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Reproduction and Development

ISSN

1040-452X

e-ISSN

—

Svazek periodika

83

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

968-982

Kód UT WoS článku

000387859100004

EID výsledku v databázi Scopus

2-s2.0-84995389660