Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43896172" target="_blank" >RIV/60076658:12520/18:43896172 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.agriculturejournals.cz/publicFiles/237458.pdf" target="_blank" >http://www.agriculturejournals.cz/publicFiles/237458.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/89/2017-CJAS" target="_blank" >10.17221/89/2017-CJAS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season

Popis výsledku v původním jazyce

Assessment of seminal oxidative stress indicators is emerging as a valuable prognostic tool in assisted reproductive technology. We investigated levels of oxidative stress caused by reactive oxygen species (ROS) and the antioxidant enzyme defence system comprising superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx) in seminal plasma (SP) of common carp Cyprinus carpio and rainbow trout Oncorhynchus mykiss over the course of the spawning season. Oxidation was determined in lipids and proteins by assessing thiobarbituric acid reactive species (TBARS) and the 2,4-dinitrophenylhydrazine carbonyl groups, respectively. Levels of SOD were assessed by the autoxidation of pyrogallol. Determination of GR and GPx was based on the rate of NADPH oxidation. We observed clear alterations in lipids and proteins over the course of the spawning season in both species. The highest levels of TBARS and CP were recorded late in the season. SOD was not significantly altered in either species. The activity of GR was higher in carp SP late in spawning compared to other times (P &lt; 0.05), while in rainbow trout, GR significantly increased (24.13 +/- 2.8 mU/mg protein) in mid-season. A significantly lower GPx activity (9.18 +/- 1.32 mU/mg protein) was found in rainbow trout SP early in the spawning season, but no significant differences in GPx were observed over the course of the season in carp. These results provide further understanding of the role of fish SP antioxidants and present new data on the oxidant and antioxidant balance in SP during the spawning season that may be of value in the development of methods for artificial reproduction of teleost species.

Název v anglickém jazyce

Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season

Popis výsledku anglicky

Assessment of seminal oxidative stress indicators is emerging as a valuable prognostic tool in assisted reproductive technology. We investigated levels of oxidative stress caused by reactive oxygen species (ROS) and the antioxidant enzyme defence system comprising superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx) in seminal plasma (SP) of common carp Cyprinus carpio and rainbow trout Oncorhynchus mykiss over the course of the spawning season. Oxidation was determined in lipids and proteins by assessing thiobarbituric acid reactive species (TBARS) and the 2,4-dinitrophenylhydrazine carbonyl groups, respectively. Levels of SOD were assessed by the autoxidation of pyrogallol. Determination of GR and GPx was based on the rate of NADPH oxidation. We observed clear alterations in lipids and proteins over the course of the spawning season in both species. The highest levels of TBARS and CP were recorded late in the season. SOD was not significantly altered in either species. The activity of GR was higher in carp SP late in spawning compared to other times (P &lt; 0.05), while in rainbow trout, GR significantly increased (24.13 +/- 2.8 mU/mg protein) in mid-season. A significantly lower GPx activity (9.18 +/- 1.32 mU/mg protein) was found in rainbow trout SP early in the spawning season, but no significant differences in GPx were observed over the course of the season in carp. These results provide further understanding of the role of fish SP antioxidants and present new data on the oxidant and antioxidant balance in SP during the spawning season that may be of value in the development of methods for artificial reproduction of teleost species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Animal Science : Živočišná výroba

ISSN

1212-1819

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

78-84

Kód UT WoS článku

000422902200005

EID výsledku v databázi Scopus

2-s2.0-85040923828