FRAP & FLIP: Two sides of the same coin?

Kód výsledku v IS VaVaI

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RIV/61388971:_____/15:00443270 RIV/67985807:_____/15:00443270

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

FRAP & FLIP: Two sides of the same coin?

Popis výsledku v původním jazyce

The aim of this study is to point out the similarity and differences of data processing based on either FRAP (Fluorescence Recovery After Photobleaching) or FLIP (Fluorescence Loss In Photobleaching) experimental techniques. The core idea, closely related to the sensitivity analysis, is based on discerning between relevant and irrelevant data. Presented mathematical model allows to visualize the mutual relation between the FRAP and FLIP methods. The whole concept resides in the processing of full spatio-temporal data instead of the space averaged time series (FRAP recovery curves). The method theoretically confirms the empirical knowledge, that the mobility of fluorescent molecules can be determined with both FRAP and FLIP methods (using the full data approach). Our analysis, based on the idealized theoretical case study, supports the conclusions of our recent experiments and thus it validates the reliability of our new approach. The presented finding are expected to be reflected into experimental protocol setup as well as we will continue working on the further enhancing the method of parameter identification.

Název v anglickém jazyce

FRAP & FLIP: Two sides of the same coin?

Popis výsledku anglicky

The aim of this study is to point out the similarity and differences of data processing based on either FRAP (Fluorescence Recovery After Photobleaching) or FLIP (Fluorescence Loss In Photobleaching) experimental techniques. The core idea, closely related to the sensitivity analysis, is based on discerning between relevant and irrelevant data. Presented mathematical model allows to visualize the mutual relation between the FRAP and FLIP methods. The whole concept resides in the processing of full spatio-temporal data instead of the space averaged time series (FRAP recovery curves). The method theoretically confirms the empirical knowledge, that the mobility of fluorescent molecules can be determined with both FRAP and FLIP methods (using the full data approach). Our analysis, based on the idealized theoretical case study, supports the conclusions of our recent experiments and thus it validates the reliability of our new approach. The presented finding are expected to be reflected into experimental protocol setup as well as we will continue working on the further enhancing the method of parameter identification.

Druh

D - Stať ve sborníku

CEP obor

BA - Obecná matematika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)

ISBN

978-3-319-16479-3

ISSN

0302-9743

e-ISSN

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Počet stran výsledku

12

Strana od-do

444-455

Název nakladatele

Springer Dordrecht

Místo vydání

Dordrecht

Místo konání akce

Granada; Spain

Datum konání akce

15. 4. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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