Ovarian structure protein 1: A sensitive molecular biomarker of gonadal intersex in female Japanese medaka after androgen exposure

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888653" target="_blank" >RIV/60076658:12520/15:43888653 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/etc.3032/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/etc.3032/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/etc.3032" target="_blank" >10.1002/etc.3032</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ovarian structure protein 1: A sensitive molecular biomarker of gonadal intersex in female Japanese medaka after androgen exposure

Popis výsledku v původním jazyce

Intersex in gonochoristic fish can be induced after exposure to androgens and estrogens. The main objective of the present study was to identify biomarkers that would be predictive of intersex in Japanese medaka (Oryzias latipes) after exposure to synthetic hormones. First a gene was identified, ovarian structure protein 1 (osp1), with strong female-specific expression during gonadal differentiation. The authors hypothesized that osp1 expression would decrease to male levels in females after the exposure of larvae (15-25 d postfertilization [dpf]) to 17-trenbolone (TRB; 5ng/L) and would increase to female levels in males exposed to 17-ethinylestradiol (EE2; 5ng/L) and that gonadal intersex would be induced later in life (60 dpf). Tissue distribution and cellular localization of OSP1 was investigated using Western blot and immunohistochemistry. The results indicate that this exposure regime delays testicular maturation in males and development of ovarian intersex in females. Although de

Název v anglickém jazyce

Ovarian structure protein 1: A sensitive molecular biomarker of gonadal intersex in female Japanese medaka after androgen exposure

Popis výsledku anglicky

Intersex in gonochoristic fish can be induced after exposure to androgens and estrogens. The main objective of the present study was to identify biomarkers that would be predictive of intersex in Japanese medaka (Oryzias latipes) after exposure to synthetic hormones. First a gene was identified, ovarian structure protein 1 (osp1), with strong female-specific expression during gonadal differentiation. The authors hypothesized that osp1 expression would decrease to male levels in females after the exposure of larvae (15-25 d postfertilization [dpf]) to 17-trenbolone (TRB; 5ng/L) and would increase to female levels in males exposed to 17-ethinylestradiol (EE2; 5ng/L) and that gonadal intersex would be induced later in life (60 dpf). Tissue distribution and cellular localization of OSP1 was investigated using Western blot and immunohistochemistry. The results indicate that this exposure regime delays testicular maturation in males and development of ovarian intersex in females. Although de

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-34049P" target="_blank" >GP13-34049P: Toxikologická proteomika: Potenciál pro identifikaci nových biomarkerů samčí plodnosti.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental toxicology and chemistry

ISSN

0730-7268

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

2087-2094

Kód UT WoS článku

000360500600023

EID výsledku v databázi Scopus

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